Sawdust cultures of Lepista nuda

민자주방망이버섯 (Lepista nuda)의 톱밥배양

  • Lee, Sang-Sun (Department of Biological Science and Education at the Graduate School, Korean National University of Education) ;
  • Choi, Kyoung-Jin (Department of Biological Science and Education at the Graduate School, Korean National University of Education) ;
  • Oh, Chang-Ho (Department of Biological Science and Education at the Graduate School, Korean National University of Education)
  • 이상선 (한국교원대학교, 대학원 생물교육학과) ;
  • 최경진 (한국교원대학교, 대학원 생물교육학과) ;
  • 오창호 (한국교원대학교, 대학원 생물교육학과)
  • Published : 1996.12.30

Abstract

An isolate of Lepista nuda was cultivated on the solid media consisted of various cereal grains or those of the acacia sawdust mixed with other cereal grains. The mycelia grew well on the solid cultures made from millet cereals, but did not on those from sorghum or corn cereals. Thus, the millet and the acacia sawdust, as the solid media, were employed for the next experiments related to mycelial growths. For growth of this isolate, it was shown that the nitrogen source is needed for those of millet. Also, the rice bran or minerals were considered to be needed for the acacia sawdust. As minerals, however, the rice bran is shown as the best additive in the solid cultures of the acacia sawdust. The mycelia of L. nuda were cultivated directly through the solid cultures. Their growths were measured' by the production of carbon dioxide with gas chromatography.

민자주방망이버섯(Lepista nuda)의 분리균을 여러 가지 곡물에서 키운 결과, 조에서는 잘 자랐으나, 수수에서는 잘 자라지 않았다. 이들 중에서 조를 선택하여, 여러 가지 유기물 혹은 금속 첨가물을 첨가한 실험 결과 조배지에서는 질소 성분이 필요하다는 것이 밝혀졌다. 이와 반면에 우리 주변에 흔히 있는 아카시나무 톱밥을 이용한 배지에서는 질소성분과 무기염류의 효과가 여러 가지로 나타난다. 그러나 유기물로서는 흔히 사용되는 미강이 가장 좋은 첨가물로 밝혀졌다. 여기서의 연구는 고형배양을 통하여 직접 균사를 배양하였기 때문에 그 의의가 크다고 하겠다.

Keywords

References

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