한국균학회지 (The Korean Journal of Mycology)

한국균학회 (The Korean Society of Mycology)
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DNA 다형성 분석에 의한 느타리버섯 단포자 분리주의 유전적 변이성

Genetic Variability of Pleurotus ostreatus Monospore Isolates by Random Amplified Polymorphic DNA Analysis

  • Song, Yeong-Jae (Department of Agronomy, Kyung Hee University) ;
  • Jeong, Mi-Jeong (Division of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Beom-Gi (Division of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Rho, Yeong-Deok (Department of Agronomy, Kyung Hee University) ;
  • Ryu, Jin-Chang (Division of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Yoo, Young-Bok (Division of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA)
  • 발행 : 1996.09.30
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초록

식품으로서의 가치가 증가하고 있는 느타리버섯(Pleurotus ostreatus) 이핵체와 이것의 자실체로부터 얻은 35 단포자분리균주의 핵산연쇄중합반응에 의한 DNA 다형성을 분석하여 몇가지 유전형질과의 관계를 검토하였다. 느타리의 300개 단포자분리균주중에서 다시 이차로 분리한 35균주의 균총생장 속도를 분석한 결과 4균주는 모균주와 유사한 경향을 나타내었고 느린 것, 아주 느린 것으로 나눌 수 있었다. 26개의 Primer를 사용한 RAPD 분석에서 모균주와 단포자분리균주간에 형성된 DNA 다형성 밴드는 345개로, 밴드의 크기는 $0.1{\sim}4.0Kb$였다. Primer J(OPA-01)와 W(OPB-04)는 균주간에 많은 차이를 보였고, 36-MI 103균주는 거의 모든 primer에서 다른 균주와 차이를 나타냈다. RAPD 분석에 의한 모균주와 단포자분리주의 유전유사도는 36-MI 103을 제외하고 약 73% 정도의 유전유사성을 보였으며, 36-MI 103 균주와 다른 분리균주간의 유사도는 49%로 낮았다. 모균주 및 단포자 분리균주들의 유전유사도는 균사 생장속도와 다소 상관이 있는 4개 군으로 나눌 수 있었는데 I군은 이핵성 모균주, II군은 빠른 생장의 단핵주, III군은 중간 및 느린 생장속도의 단핵주, IV군은 아주 느린 생장속도의 단핵주였다. 그러나 교배형과 유연관계는 상관관계가 없는 것으로 나타났다.

This study was carried out to obtain data concerning the genetic variability of Pleurotus ostreatus. Monospores of P. ostreatus were isolated and cultured to estimate differences in the rate of mycelial growth and genetic similarity among the isolates. Although the overall growth rates were slow compared to their parental dikaryon except 2-MI 4, significant differences in the rate of mycelial growth were observed among isolates. Random amplified polymorphic DNA (RAPD) analysis using twenty six random primers showed 345 polymorphic DNA bands from 35 monospore isolates and their parental dikaryon. DNA bands ranged from 0.1 to 4.0 Kb in size. Most various polymorphic DNA bands within monospore isolates were obtained when we used J (OPA-01) or W (OPB-04). The 36-MI 103 showed totally different band patterns from those of the others. RAPD analysis revealed that there is approximatly 75% genetic similarity between monospore isolates and their parental dikaryon except 36-MI 103, which showed only 49% genetic similarity. In addition, genetic similarity degrees were classified into four groups: I (parental dikaryon), II (fast growing type), III (moderate growing type), and IV (slow growing type). However, there is no correlation between mating type and mycelial growth rates.

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참고문헌

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