The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지

Changes in activities of protease, phenoloxidase and cellulase during mycelium growth of Pleurotus ostreatus in sawdust cultures

톱밥배양한 느타리버섯 균사생장시 생산되는 각종 효소변화

  • Chang, Hyun-You (Division of Applied Microbiology, Agricultural Sciences and Technology Institute, RDA) ;
  • Kim, Gwang-Po (Division of Applied Microbiology, Agricultural Sciences and Technology Institute, RDA) ;
  • Cha, Dong-Yeul (Division of Applied Microbiology, Agricultural Sciences and Technology Institute, RDA)
  • 장현유 (농업과학기술원 응용미생물과) ;
  • 김광포 (농업과학기술원 응용미생물과) ;
  • 차동열 (농업과학기술원 응용미생물과)
  • Published : 1996.06.30
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Abstract

Effects of various kinds of sawdusts, supplements and culture conditions on activities of several enzymes such as protease, phenoloxidase and cellulase produced from mycelium of P. ostreatus grown on sawdust medium were studied and the results are as follows; Higher specific activity of these enzymes was observed when oak tree sawdust and poplar tree sawdust were supplemented with rice bran or wheat bran at rate of 30%, 20% and 10% in total volume respectively. Higher total activities of protease, phenoloxidase and cellulase were observed at 70% of the moisture contents of culture media, while lower activity of these enzymes was observed with 40% moisture contents of sawdust culture medium. The pH 4 and 9 of the sawdust media appeared to be optimum pH for the. production of protease while pH 5 and 7 were optimal for the production of phenoloxidase. The pH 6 of the sawdust medium was optimal for the production of cellulase. The optimum incubating temperature for the production of protease, phenoloxidase and cellulase was $25^{\circ}C$. Higher total activities of protease and phenoloxidase were observed when culture medium was added with wood vinegar at the control, and 0.5% for cellulase.

느타리버섯 균사를 톱밥에 배양할 때 분비되는 여러 가지 extracellular enzyme중 pretense, phenoloxidase, cellulase가 배양조건을 달리하였을 때 변화양상을 요약하면 먼저 톱밥의 종류에 따라서는 참나무톱밥에 비해 포플라톱밥이 specific activity가 높은 경향이었다. 첨가제 종류에 따라서는 밀기울에 비해 미강이 약간 높았으며 혼합비율이 30, 20, 10%순으로 높았다. 수분함량은 3가지 효소 모두 70%에서, 배지의 PH에 따라서 protease는 pH 4와 9, cellulase는 pH6, phonoloxidase는 pH 5와 7, 배양온도는 3가지 효소 모두 $25^{\circ}C$, 목초액 농도에 따른 pretense와 phonoloxidase는 무처리가 처리보다 총활성이 높았으며, cellulase는 0.5%에서 총활성이 가장 높았다.

Keywords

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