The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Studies on immunomodulating function of components separated from higher fungi

고등균류 균사체의 면역조절 기능성에 관한 연구

  • Bae, Man-Jong (Dept of Food Science, Kyungsang University) ;
  • Park, Mu-Hee (Dept. of Food Science and Technology, Yeungnam University) ;
  • Lee, Jae-Sung (Dept. of Food Science and Technology, Yeungnam University)
  • Published : 1996.06.30
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Abstract

To find compounds of immunomodulating and anti-allergic function, effects of protein-bound polysaccharides extracted from Phellinus igniarius (PI), Fomitella fraxinea (FF) and Agrocybe cylindracea (AC) on hemagglutinin titer (HA), hemolysin titer (HY), plaque forming cell (PFC), rosette forming cell (RFC) and phagocytosis were investigated in BALB/C mice. The oral administration of the protein-bound polysaccharides of PI, FF and AC for 10 days resulted in the enhanced phagocytic activity of peritoneal exudate cells (PEC), spleen cells (SC) and blood lymphocyte cells (BLC). Moreover, PI showed the activating effect on the phagocytosis of PEC and AC in SC. In the experiment of PFC and RFC, the results of the experimental group which was given each samples as compared to the control group, showed the enhanced level of activity such as PI 130%, FF 90% and AC 70%. Generally, HY and HA showed from ten to hundred times of level in each sample groups, as compared to the control group.

고등균류 균사체에 대한 면역조절기능과 항알레르기성 물질을 탐색하기 위해서 우선 단백다당류를 추출 회수해서 몇 가지 관련된 실험을 수행하였다. 면역감수성이 우수한 BALB/C 마우스를 통해서 적혈구 응집과 용혈반응, 항체생산능을 확인하기 위해서 PFC와 RFC실험, 마크로파아지와 임파구 세포들의 탐식능력 실험을 수행한바 다음과 같은 결과를 얻었다. 1. 탐식능의 결과는 상황버섯(Phellinus igniarius), 장수버섯(Fomitella fraxinea), 버들송이(Agrocybe cylindracea), 균사다당체 모두가 복강탐식세포, 말초임파구, 비장의 식세포에 대해서 유의성 있는 식작용을 확인했다 상황은 복강식세포, 버들송이는 비장식세포에 대해서 식작용을 활성화시키는 결과를 나타냈다. 2. PFC와 RFC의 실험결과에서도 대조군에 비해 시료를 투여한 실험군에서 면역세포가 상황 130%, 장수 90%, 버들송이 70% 정도로 증강된 활성을 보였다. 3. 용혈소가와 응집소가는 전반적으로 대조군에 비해 균사단백다당체가 $2{\sim}3$배의 높은 값을 보였다.

Keywords

References

  1. Gann. v.67 Differentiation of host-mediated antitumor agents from mitotic poisons by the antitumor foot-ped reaction in Ehrlich carcinoma-ddY mouse system Abe, S.;Ohuma, M.;Yamazaki, M.;Mizuuno, D.
  2. Cancer Res. v.30 Fractionation and purification of the polysaccharide with marked antitumor activity, especially lentinan from Lentinus edodes Chihara, G.;Hamuro, J.;Maeda, Y.Y.;Arai, Y.;Fukuoka, F.
  3. Kor. J. Mycol v.16 no.3 Studies on constituents of higher of Korea(LVII). Comparison of various antitumor constituents of Coriolus versicolor Cho, H.J.;Shim, M.J.;Choi, E.C.;Kim, B.K.
  4. Immunology v.14 Future improvements in the plaque technique for detecting single antibody-forming cell Cunningham, A.J.;Szenbirg, A.
  5. J. Allergy. Clin. Immunol v.92 no.4 Immunodeficiency and other clinical immunology Giuseppe, M.C.;Francesco, F.;Valerio, D.O.;Riccardo, P.;Nera, A.;Bibbiana, D.S.;Giuliano, C.;Carlo, A.P.
  6. Kor. J. Mycol v.10 no.4 Studies on constituents of higher fungi of Korea (XXXIII). Antitumor components of trametes sanguinea Hong, W.B.;Chung, K.S.;Woo, M.S.;Kim, B.K.
  7. Kor. J. Mycol v.18 no.2 Studies on constituents of higher fungi of Korea(LXVII). Antitumor components of the basidiocarp of Ganoder lucidum Hyun, J.W.;Choi, E.C.;Kim, B.K.
  8. Method in Immunology Justine, S.G.;Natalie, E.C.;Dieter, H.S.
  9. Method in Immunology Justine, S.G.;Natalie, E.C.;Dieter, H.S.
  10. Method in Immunology Justine, S.G.;Natalie, E.C.;Dieter, H.S.
  11. Arch. Pharm. Res. v.2 Studies on the constituents of higher fungi of Korea(XXIII), antineoplastic activities of Coriolus versicolor(L. ex Fr.) Quel., Pleurotus ostreatus(Fr.) Kummer and Lentinus edodes(Berk.) Sing Kim, B.K.;Park, E.K.;Shim, M.J.
  12. Immunology v.47 Immunostimulation polysaccharides separated from hot water extract of Anpelica acatiloba Kitagawa (Yamato Tohki) Kumazawa, Y.;Mizunoe, K.;Otsuka, Y.
  13. Kor. J. Mycol. v.20 no.3 Studies on constituents of higher fungi of Korea(LXXIII). Antitumor components of cultured mycelia of Paxillus atromentosus Kwag, S.D.;Bok, J.W.;Hyun, J.W.;Chol, E.C.;Kim, B.K.
  14. J. Mycol. v.9 no.1 Studies on antitumor components of korean basidiomycetes (II). Antitumor components of Schizophyllum commune and Auricularia auricula-judae Lee. S.A.;Chung, K.S.;Shim, M.J.;Choi, E.C.;Kim, B.K.
  15. Gann v.66 Restoraton of antibody-forming capacities by PS-K in tumor-bearing mice Nomota, K.;Yoshikumi, C.;Matsunage, K.;Fujii, T.;Takeya, K.
  16. Prodution of protein-bound polysaccharides by Coriolus versicolor, Lentinus edodes Park, K.S.
  17. Quel. Kor. J. Mycol. v.20 no.1 Extraction and separation of protein-bound polysaccharide produced by Coriolus versicolor(Fr) Park, K.S.;Lee, J.Y.;Lee, S.J.;Kim, S.H.;Lee, J.S.
  18. Science v.23 Clavacin a new antitumor agent Roland, J.F.;Chielewicz, Z.F.;Weiner, B.A.;Gross, A.M.;Boenning, O.P.;Bardos, T.J.; Reilly, H.C.;Sugiura, K.;Stock, C.C.;Lucas, E.H.;Byerrum, R.U.;Stevens, J.A.
  19. Gann v.5 Protein-bound polysaccharide preparation, PS-K, effective against mouse sarcoma-180 and ascites hepatoma AH-13 hy oral use Tsukagosi, S.;Ohashi, F.
  20. Quel. Carbohydrate Res. v.140 Isolation, purification and structure of components from acidic polysaccharides of Pleurotus ostreatus(Fr.) Yoshioka, Y.;Emori, M.;Ikekawa, T.;Fukuoka, F.
  21. Carbohydrate Res. v.43 Antitumor polysaccharide from P.ostreatus (Fr.) Quel. isolation and structure of $\beta$-glucan Yoshioka, Y.;Tada, R.;Saito, R.;Saito, H.;Uehara, N.;Fukuoka, F.
  22. 營養學雜誌 v.48 no.2 食物 알레르기와 그 臨床 馬場實
  23. 化學과 生物 v.25 食品 allergy의 低減化 上野川修一
  24. 蛋白質·核酸·酵素 v.40 no.4 마이크로페이지의 리포폴리사카라이드의 受容體 西島正弘
  25. 炎炳 v.4 no.4 貪食能 側定法-마우스 腹腔細胞 및candida parapsilosis(CP)を用ぃて- 小松晴弘;小野尙産;安倍千之
  26. 營養學雜誌 v.45 no.6 食物 알레르기 松村龍雄
  27. 生體防御의 成立 野本龜久雄
  28. 食品과 生體防禦 村上浩紀;上野天修一
  29. 管養學雜誌 v.42 no.1 管養과 生體防衛 板本元子
  30. 管養과 生體防禦 板本元子;今泉勝己;菅野道廣