BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Identification and Purification of a Normal Rat Liver Plasma Membrane Surface Protein which Disappears after Chemical Carcinogenesis

  • Kim, Min-Young (Hanhyo Institutes of Technology) ;
  • Lee, Myung-Kyu (Protein Engineering Research Group, Korea Research Institute of Bioscience and Biotechnology) ;
  • Hahm, Kyung-Soo (Protein Engineering Research Group, Korea Research Institute of Bioscience and Biotechnology)
  • Received : 1995.06.10
  • Published : 1995.11.30
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Abstract

The electrophoretic patterns of plasma membrane surface proteins of normal rat liver cells and rat hepatomas were compared in 10% non-denaturing and 7-15% gradient non-denaturing gel. Chemical carcinogens, 2-Me DAB (2-methyl-4-dimethylaminoazobenzene) and DENA (diethylnitrosamine), were used to induce hepatoma in rats. One protein which disappeared in hepatoma was identified in normal rat liver by non-denaturing gel electrophoresis. Rabbit antisera were raised against this specific protein, and the protein was purified by Sephacryl S-200 column and immunoaffinity chromatography using the purified antibody. The purified protein showed two bands of molecular weights approximately 50 $kD_{\alpha}$ and 52 $kD_{\alpha}$ by SDS-polyacrylamide gel electrophoresis, which reacted specifically with the antibody. However only one band was observed in non-denaturing gel and also in isoelectric focusing with a pI value of 6.6. This study showed the existence of an unique protein on the plasma membrane surface of normal rat liver cells which disappeared in rat hepatomas induced by chemical carcinogens.

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