Tuberculosis and Respiratory Diseases
- Volume 42 Issue 6
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- Pages.823-830
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- 1995
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- 1738-3536(pISSN)
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- 2005-6184(eISSN)
Immunocytochemical Study on the Change of the Activated T Cells in Peripheral Blood of the Pulmonary Tuberculosis Patients
폐결핵환자의 말초혈액에서 Activated T Cell의 변화
- Ryu, Gyeong-Ryeol (Department of Internal Medicine, College of Medicine, Gyeong Sang National University) ;
- Park, Une-Sook (Department of Internal Medicine, College of Medicine, Gyeong Sang National University) ;
- Park, Chong-Hwa (Department of Internal Medicine, College of Medicine, Gyeong Sang National University) ;
- Jeoung, Pan-Joon (Department of Internal Medicine, College of Medicine, Gyeong Sang National University) ;
- Hwang, Young-Sil (Department of Internal Medicine, College of Medicine, Gyeong Sang National University) ;
- Lee, Yang-Jae (Department of Anatomy, Seoul National University College of Medicine) ;
- Chang, Ga-Yong (Department of Anatomy, Seoul National University College of Medicine)
- 류경렬 (경상대학교 의과대학 내과학교실) ;
- 박은숙 (경상대학교 의과대학 내과학교실) ;
- 박종화 (경상대학교 의과대학 내과학교실) ;
- 정판준 (경상대학교 의과대학 내과학교실) ;
- 황영실 (경상대학교 의과대학 내과학교실) ;
- 이왕재 (서울대학교 의과대학 해부학교실) ;
- 장가용 (서울대학교 의과대학 해부학교실)
- Published : 1995.12.31
Abstract
Background: It has been found that Helper T cells in the peripheral blood are decreased in the cell mediated immunity in the pulmonary tuberculosis. But it has not been confirmed yet that only decrease in number of cells which has phenotype in the peripheral blood is defined to decrease in cell mediated immunity. The immunocytochemical study was performed to observe the change of the percentage of T-lymphocytes with their subsets and activated T cells in the peripheral blood of pulmonary tuberculosis and to know how many T cells would be activated, relative to resting cells in the peripheral blood. Methods: The peripheral blood obtained from twenty two patients and ten healthy controls were smeared on the gelatin coated slide glass prepared for of mononuclear cells. The double bridge technique of alkaline phosphatase-antialkaline phosphatase(APAAP) method was used. As the primary antibodies,
연구배경: 결핵감염시 세포매개성 면역반응이 관여하여 말초혈액내 조력 T 세포가 감소하는 것으로 알려저왔다. 그러나 말초혈액내 표지물질을 가진 세포의 수적 감소가 세포매개성 면연반응의 저하라고 할 수있지 여부가 앞으로 해결해야 될 문제인 것으로 지적되고 있다. 이에 저자들은 폐결핵 환자들의 말초혈액에서 활성화된, 또는 활성화 되고 있은 세포의 수적변화를 관찰하기 위하여 본 연구를 시행하였다. 방법: 객담 결핵균 양성인 폐결핵 환자 22명을 대상으로 말초혈액에서 IL-2R, VLA-1, TLiSAI의 활성화 표지에 대한 단세포군 항체를 면역조직 화학법으로 측정하였다. 결과: 1) 폐결핵 환자의 말포혈액에서