Histological Observation on the Seasonal Changes of Distribution of Muscle Components in Hibernant Fish - 2. Distributional Changes of Carbohydrate, Protein and Lipid Components in the Muscle Tissues of Mudskipper, Boleophthalmus pectinirostris

동면 어류의 시기별 근육성분 분포에 관한 조직학적 관찰 - 2. 짱뚱어(Boleophthalmus pectinirostris) 근육조직중 탄수화물, 단백질 및 지방질 분포의 변화

  • Park, Il-Woong (Dept. of Food Science and Technology, Chonbuk Sanup University) ;
  • Hong, Jai-Sik (Dept. of Food Science and Technology, Chonbuk National University) ;
  • Lee, Keun-Kwang (Dept. of Biology, Kunsan National University) ;
  • Kim, Jong-Bae (Dept. of Fisheris processing, Kunsan National University) ;
  • Kang, Kui-Hwan (Dept. of Food Science and Technology, Chonbuk Sanup University) ;
  • Kim, Hyun-Ouk (Dept. of Molecular Biology, Wonkwang University)
  • 박일웅 (전북산업대학교 식품공학과) ;
  • 홍재식 (전북대학교 식품공학과) ;
  • 이근광 (군산대학교 생물학과) ;
  • 김종배 (군산대학교 수산가공학과) ;
  • 강귀환 (전북산업대학교 식품공학과) ;
  • 김현옥 (원광대학교 분자생물학과)
  • Published : 1995.12.30

Abstract

The seasonal changes in the muscle components of hibernant fish, amphibious mudskipper (Boleopthalmus pectinirostris), caught during the period between June 1993 and April 1994 were studied. The distributional changes of its major components in each muscle tissues was also observed before and after spawning and hibernation. Moisture content was the lowest after spawnig season and the highest just after hibernation, but crude protein was the highest after spawning sea-son. Crude lipid in female was the highest before spawning season, while in male it was the highest after spawning season, but both of female and male the lowest just after hibernation. Carbohydrate content in female and male showed the highest value just before hibernation and tended to decrease thereafter. In case of mudskipper, dyeing distribution was more or less spread in almost all of the tissues after spawning season but showed nearly no difference just after hibernation with the exception of small increase in dyeing degree of muco layer and epidermis layer. Investigation of mudskipper muscle tissue through the method of sudan black B dyeing yielded the results as follows ; lipid component in mudskipper in seasons before and after spawning season was percieved as mainly distributed in muco layer, epidermis and hypodermis layer respectively and also percieved a little between hypodermis subscutoneus cells. In season just before hibernation, it prevailed in almost all of the tissues, contrary to the weakness in its distribution degree just after hibernation.

짱뚱어를 대상으로 산란기 전(6월), 산란기 후(9월)와 동면 직전(11월), 동면직후(4월)에 있어 자웅(雌雄) 각각에 대한 근육성분의 조성을 분석하고 주요 육(肉)성분에 대한 조직내 분포를 검토한 결과는 다음과 같다. 비만도(肥滿度)는 암컷은 산란기 전, 숫컷은 산란기 후가 다소 높았으나 동면후는 가장 낮았다. 수분 함량은 자웅(雌雄) 모두가 산란기 후가 가장 적으나 동면 직후가 가장 많았고 조단백질은 산란기 후에, 조지방은 암컷은 산란기전, 수컷은 산란기 후가 가장 많아 수분과 조단백질 사이 (암컷, 수컷), 그리고 수분과 조지방 함량 사이(수컷)의 변화양상이 대체로 상보적(相補的) 관계를 이루었고 탄수화물함량은 자웅(雌雄) 모두가 동면직전에 다소 많았고 동면직후에 가장 적었다. 근육조직의 PAS염색강도가 산란기 전에는 비교적 근육조직 전반에 걸쳐 높게 나타났고 동면직후는 점액층과 표피층이 다소 높은 경향이었으나 뚜렷한 차이는 인지할 수 없었다. Sudan black B 염색결과, 지방질 염색반응이 산란기 전과 후에는 주로 점액층과 표피및 피하층에 나타났고, 피하조직 세포 사이에서도 다소 인지되었다. 동면 직전과 직후에는 조직전반에 걸쳐 염색반응이 산재된 경향이었으나 동면직후는 그 정도가 다소 적은 편이었다.

Keywords