Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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AN EXPERIMENTAL STUDY ON THE REACTION OF PULPAL CELLS TO THE IMPLANTED DEMINERALIZED BONE MATRIX

탈회골기질 이식에 대한 치수세포의 반응에 관한 실험적 연구

  • Kim, Sun-Hun (Dept. of Oral Anatomy, College of Dentistry, Chonnam National University) ;
  • Kim, Min-Seok (Dept. of Oral Anatomy, College of Dentistry, Chonnam National University) ;
  • Oh, Won-Mann (Dept. of Conservative Dentistry, College of Dentistry, Chonnam National University)
  • 김선헌 (전남대학교 치과대학 구강해부학교실) ;
  • 김민석 (전남대학교 치과대학 구강해부학교실) ;
  • 오원만 (전남대학교 치과대학 보존학교실)
  • Published : 1995.09.10
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Abstract

Implantation of demineralized bone matrices was done into the amputated pulp in vivo and sequential reaction of the pulpal ectomesenchymal cells was observed. The bone matrices, obtained from cat long bone were crushed into below $700{\mu}m$, demineralized with 0.5N HCl and allografted into pulp of molar teeth. At seven days after implantation many undifferentiated mesenchymal cells aggregated near the matrices in the pulpal tissue. At fourteen days after implantation, the cells differentiated into preosteoblast-like cells which have secretory cell characteristics. At one or two months after implantation osteoid tissue was formed. The cells, which are located at the surface of the tissue, contained abundant dilated rough endoplasmic reticulum, Golgi apparatus and secretory granules in the cytoplasm. The matrix of the tissue has less collagen fibers than those in normal dentin. These results suggest that the interaction of pulpal mesenchymal cells with demineralized bone matrix can be a model which induces mineralization.

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