Restorative Dentistry and Endodontics
- 제20권2호
- /
- Pages.549-576
- /
- 1995
- /
- 2234-7658(pISSN)
- /
- 2234-7666(eISSN)
연쇄구균의 세포벽 단백질이 L929 세포의 미세구조 변화에 미치는 영향에 관한 연구
THE EFFECT OF CELL WALL PROTEINS OF STREPTOCOCCUS SPECIES ON MICROSTRUCTURAL CHANGES OF L929 CELLS
- Oh, Sae-Hong (Department of Conservative Dentistry, College of Dentistry, Wonkwang University) ;
- Im, Mi-Kyung (Department of Conservative Dentistry, College of Dentistry, Wonkwang University)
- 발행 : 1995.09.10
초록
Bacteria are one of the most important causative agents of the pulpal and periapical diseases. Streptococci are one of the most frequently isolated facultative anarerobic bacteria in the infected root canals. Bacterial cell wall components have a direct effect in the pathogenesis of the pulpal and periapical infections. Hyaluronidase produced by bacteria has been implicated in dissemination of the diseases. The purpose of this study was to evaluate the effect of cell wall extract of streptococci on the L929 cells using inverted microscope and the transmission electron microscopy (TEM). Hyaluronidase production of streptococcal strains were investigated to determine the correlation between the severity of cell damage and the activity of enzymes. Bacterial cell wall extracts of S. sanguis, S. mitis and S. uberis isolated from infected root canals and ATCC type strains of S. mutans (ATCC 10449) and E. faecalis (ATCC 19433) were prepared by sonication and confirmed with SDS-PAGE. Silver stain of SDS-PAGE of sonic extract was efficient at
키워드