Journal of Periodontal and Implant Science
- 제25권3호
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- Pages.497-517
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- 1995
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- 2093-2278(pISSN)
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- 2093-2286(eISSN)
Superoxide Dismutase가 치주인대 세포에 미치는 면역세포학적 연구
IMMUNOCYTOCHEMICAL STUDY OF THE EFFECT OF SUPEROXIDE DISMUTASE ON THE PERIODONTAL LIGAMENT CELLS
- 강현구 (원광대학교 치과대학 치주과학교실) ;
- 강정구 (원광대학교 치과대학 치주과학교실) ;
-
유형근
(원광대학교 치과대학 치주과학교실) ;
-
신형식
(원광대학교 치과대학 치주과학교실)
- Kang, Hyun-Koo (Department of Periodontology, College of Dentistry, Wonkwang University) ;
- Kang, Jung-Ku (Department of Periodontology, College of Dentistry, Wonkwang University) ;
-
Yoo, Hyung-Keun
(Department of Periodontology, College of Dentistry, Wonkwang University) ;
-
Shin, Hyung-Shik
(Department of Periodontology, College of Dentistry, Wonkwang University)
- 발행 : 1995.11.30
초록
The cells associated with normal defense mechanism in inflammation release free oxygen radicals, hydroxy radicals, and various protease, all of which can damage the surrounding cells(fibroblasts) and matrix molecules(collagen). The objective of this study was to evaluate the effects of "scavenger" enzyme, superoxide dismutase(SOD). to periodontal ligament (PDL) cells. Human PDL cells were cultured from the teeth extracted for non-periodontal reason. Cultured PDL cells in vitro were treated with SOD and LPS according to dosage and culture times. Cellular activity was exaimed by Microtitration(MTT) assay. The quantitative expression of cellular proliferation by proliferating cell nuclear antigen(PCNA), collagen type I and fibronectin by indirect immunocytochemically stain in PDL cells were done. The results were as follows: 1. As only SOD treated group at 2 and 3 days, PDL cell activity was significantly increased at more than 150U(P<0.05). 2. When LPS(0.5,
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