DNA FINGERPRINTING AND SEROTYPING OF ACTINOBACILLUS ACTINOMYCETEMCOMITANS ISOLATED FROM PERIODONTAL PATIENTS

Actionbacillus actinomycetemcomitans의 임상분리 혈청형에 따른 유전자 지문 양상에 관한 연구

  • Heo, Kyung-Kee (Dept. of Periodontology, College of Dentistry, Pusan National University) ;
  • Kim, Sung-Jo (Dept. of Periodontology, College of Dentistry, Pusan National University) ;
  • Choi, Jeom-Il (Dept. of Periodontology, College of Dentistry, Pusan National University)
  • 허경기 (부산대학교 치과대학 치주과학교실) ;
  • 김성조 (부산대학교 치과대학 치주과학교실) ;
  • 최점일 (부산대학교 치과대학 치주과학교실)
  • Published : 1995.04.30

Abstract

54 clinical isolates of Actinobacillus actinomycetemcomitans showed distinct hybrdization patern(DAN fingerprinting patterns) when the bacterial DNA were hybridized with randomly cloned 4.7 - kb DNA probe. The frequency of the genotypic distribution demonstrated that type C was the most prevalent genotype, the next being D, NT, A, B, and E in the descending order. The most prevalent serotype was serotype c, the next being a, nd, and b in the descending order. It was noted that the one serotype can represent more than two different genotypes and that multiple genotypic variants can also exist in the periodontal pockets within the sam subject.

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