Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cloning of Autonomously Replicating Sequence from Phaffia rhodzyma

  • Chun, Soon Bai (Department of Microbiology, College of Natural Sciences Chonnam University) ;
  • Seung Hee Chun (Department of Microbiology, College of Natural Sciences Chonnam University)
  • Published : 1995.12.01
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Abstract

A Phaffia rhodozyma chromosomal fragment (approximately 3.8 kb) capable of functioning as an origin for the replication of a kanamycin resistance ($Km^r$) plasmid in S. cerevisiae was isolated by the use of origin search plasmid, pHN134. In S. cerevisiae, transformation frequencies using the plasmid pHN134 containing an autonomously replicating sequence of P. rhodozyma was 450-580 CFU/$\mu g$ DNA. The stability of the recombinant plasmid were 16-19$\%$.

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