Journal of Microbiology and Biotechnology
- Volume 5 Issue 6
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- Pages.319-323
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- 1995
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Phenylalanyl-2-Sulfanilylglycine as Substrate for Leucine Aminopeptidase Assay
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Hwang, Se-Young
(Department of Biotechnology, Korea University) ;
- Cho, Suk-Young (Department of Biotechnology, Korea University) ;
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Yoo, Ick-Dong
(Korea Research Institute of Bioscience and Biotechnology, KIST)
- Published : 1995.12.01
Abstract
A chromogenic mimic of phenlyalanyl-dipeptide, L-phenylalanyl-L-2-sulfanilylglycine (PSG), was synthesized and examined for its usability in leucine aminopeptidase (LAP) assay. The enzyme activity was easily determined by measuring the amount of diazotized adduct of sulfanilic acid released upon hydrolysis of PSG (