Journal of Microbiology and Biotechnology
- Volume 5 Issue 5
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- Pages.302-304
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- 1995
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
A New HPLC Condition for the Analysis of Aclacinomycins A and Y in the Mixtures of Aclacinomycins
- Kim, Wan Seop (Department of Biology, Korea University) ;
- Won Tae Cho (Central Research Center, Dong Kook Pharmaceutical Co.) ;
- Myung Kook Kim (Central Research Center, Dong Kook Pharmaceutical Co.) ;
- Sang Ki Rhee (Applied Microbiology Research Group, Korea Research Institute of Bioscience and Biotechnology) ;
- Hak Ryul Kim (Department of Biology, Korea University) ;
- Eul Sung Choi (Applied Microbiology Research Group, Korea Research Institute of Bioscience and Biotechnology)
- Published : 1995.10.01
Abstract
As the previous HPLC method (Ogasawara et al., 1983) was described for the analysis of aclacinomycin A and some of its analogue compounds but not for aclacinomycin Y, we developed novel HPLC condition by optimizing solvent system. The newly developed solvent system allowed a complete separation of aclacinomycins Y and A, as opposed to the incomplete resolution of these two compounds in conventional method. The amounts of aclacinomycins Y and A could be accurately determined when the fermentation broth of Streptomyces lavendofoliae DKRS was analyzed by the newly developed method.