Journal of Microbiology and Biotechnology
- Volume 5 Issue 5
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- Pages.280-284
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- 1995
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Occurrence of OF494911 in the Fungal Mat formed by Surface Culture of Aspergillus niger F-580
- Chun, Hyo-Kon (Microbial Chemistry Research group, Korea Research Institute of Bioscience and Biotchnology, KIST) ;
- Chung, Myung-Chul (Microbial Chemistry Research group, Korea Research Institute of Bioscience and Biotchnology, KIST) ;
- Ko, Hack-Ryong (Microbial Chemistry Research group, Korea Research Institute of Bioscience and Biotchnology, KIST) ;
- Lee, Ho-Jae (Microbial Chemistry Research group, Korea Research Institute of Bioscience and Biotchnology, KIST) ;
- Kho, Yung-Hee (Microbial Chemistry Research group, Korea Research Institute of Bioscience and Biotchnology, KIST)
- Published : 1995.10.01
Abstract
Aspergi11us niger F-580, a potent producer of aminopeptidase M inhibitor, was isolated from the brown spots of plant leaves with a pathological trait. The inhibitory activity was found only in the fungal mat formed by surface culture of Aspergi11us niger F-580, but not in the culture supernatant or cell pellet. The inhibitor was purified from the hot water extract of this fungal mat by using chromatographies on Diaion HP-20, DEAE-cellulose, Sephadex G-l0 and YMC-ODS-AQ columns. The purified inhibitor was analyzed by UV, mass, and NMR spectroscopies, and identified as OF494911, which had been isolated as an aminopeptidase B inhibitor from Penicillium rugulosum OF4949