Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Ultrastructural Characteristics of Developmental Stages During in vitro Regeneration in Citrus junos SIEB.

유자 (Citrus junos SIEB.) 의 발생단계에 따른 미세구조적 특성

  • Published : 1995.12.01
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Abstract

In this study, the callus was induced and regenerated from the immature embryo and ultrastructural characteristics of developmental stages in Citrus junos SIEB, were investigated. The yellowish callus was induced by 5 to 6 week of culture of citrus. In proliferation callus after 6 weeks of culture, large vacuole was formed by fusion between adjacent small ones. In the non-embryogenic callus cultured for 12weeks, re-differentiated cells of callus showed the large nucleus with globular nucleus and amyloplast with large size of starches. In the embryogenic callus cltured for 14-16 weeks, the active exocytosis occurred in cells, secretory vesicles appeared on cell membrane and small particles from cytoplasm were released to intercelluar space. In the embryogenic callus cultured for 24 weeks, a sperical type of chloroplast bounded on cytoplasm by double membrane and typical grana was dispersed equally among matrix. In the normal plantlet after 26 weeks of culture, a lot of vessels and companion cells apperaed in the leaf cell of plantlet. In the normal plantlet after 30 weeks of culture, the immature leaf showed many small companion cells, sieve tubes and central vacuole. Also, the secondary vacuole protruded into the central vacuole and elongated chloroplasts near plasma membrane. In the matured plant habituated on the soil, palisada tissue composed of orderly arranged cells contained the nucleus in the center of the cell and large vacuoles on either side of the nucleus.

본 연구에서는 유자의 미성숙 배로부터 캘러스 유도와 재분화 및 발생단게별 미세구조적 특성에 관하여 연구하였다. 유자에서는 배양 5-6주 경에 담황색의 캘러스가 유도되었으며, 배양 6주경의 캘러스 세포에서는 인접하고 있는 작은 액포들이 융합하여 큰 액포를 형성하는 융합현상을 관찰하였다. 배양 12주 경에는 배발생 캘러스의 미분화 세포에서 특이하게 구형의 핵인을 가지고 있는 큰핵과 녹말로 가득차 있는 녹말체를 다수 관찰하였다. 배양 14-16주 경의 배발생 캘러스 세포에서는 활발하게 exocytosis가 일어나므로서 다수의 분배체들이 세포벽쪽에서 관찰되었으며 세포질로부터 작은 입자들이 세포 간극으로 빠져나가는 것을 볼수 있었다. 또한 배양 24주경의 배발생 캘러스에서는 발달된 커다란 구형의 엽록체들이 이중막으로 세포질과 경계되어 있었으며 기질중에 일정하게 전형적인 그라나가 분포되어 있었다. 배양 26주경의 어린 정상 식물체의 미성숙잎에서는 다수의 도관과 반세포들이 관찰되었다. 배양 30주경의 정상식물체의 미성숙잎에는 다수의 작은 반세포들과 사관 그리고 중심액포들이 나타났으며 또한 작은 제2차 액포들이 커다란 중심 액포 속으로 끼어들어가는 현상을 볼 수 있었으며 원형질막 근처에는 신장된 엽록체들이 분포되어 있었다.

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References

  1. Pflanzenphysid v.58 Untersrchungen an den spritzdrusen von pictammus albus L.Z. Aelunxen,F.;H.Arbeiter
  2. Plante v.164 Establishment and maintenance of friable, embryogenic maize callus and the involvement of S-proline Amstrong,C.L.;C.E.Green
  3. S.Bioscience v.25 Crop improvement through techniques of plant cell and tissue cultures Calson,P.S.
  4. Ann Bot. v.3 Occurence and structure cuticular wax on juice vesicles of Citrus fruits Fahn,A.;I.Shomer;I.Ben-Gera
  5. Ann. Rev. Biochem. v.48 Electron microtron microsocpic visualization of nucleic acids and of their complexes with proteins Fisher,H.W.;R.C.Willian
  6. Proc. Natl. Acad. Sci. USA v.75 Variation in mitochondrial translation products associated with male-sterile cytoplasms in maize Forde,B.G.;R.J.C Oliver;C.J.Leaver
  7. Plant Physiol. v.70 Characterization of mitrochondrial DNA in citrus Fontarnau Agustin;jose Hernandez-Yage
  8. Proc. Natl. Acad. Sci. USA v.77 Variation in mitochondrial translation products associated with male-sterile cytoplasms in maize Forde,B.G.;R.J.C Oliver;C.J.Leaver
  9. Plant Mol. Biol. v.3 Accurata processing and pseudouridylation of chloroplast tranfer RNA in a chloroplast transcription system Greeberg,B.M.;W.Gruissem;R.B.Halick
  10. Plant Cell Rep. v.6 Direct somatic embryogenesis and plant regeneration from immature embryos of hybrid sunflower(Helanthus annuus L.)on an high sucrose containing medium John,J.F.
  11. Lorea J Plant Tissue Culture v.18 The stiulation of embrogensis and plant regeneration from cultured cells of rice (Oryza sativa L.) Kim,K.M.;J.Button
  12. Plant Cell v.23 Cryopreservation in Liquid Nitrogen of Cultured Navel Orange (Citrus sinesis Osb.) Nucellar cells and subsequent plant regeneration Kobayashi,S.;A.Sakai;I.Oiyama
  13. Z. Pflanzenphysiol. v.73 The stimulation of embryogenesis and embryoid development in habituated ovular callus from the Shamouti orange(Citrus sinesis)as affected by tissue age and sucrose concentration Kochba,J.;J.Button
  14. L. Z. Pflanzenphysiol. v.111 propagation of embryogenic culture of Medicago sativa Luptto,E.
  15. J.amer.soc.Hortsic. v.116 Somatic embrogenesis and plant regeneration from leaves of Dendranthema grandiflora May,R.A.;R.N.Trigiano
  16. Korean J. Plant Tissue Culture v.20 no.4 Plant regeneration of chinese Jujube (Zizyphus jujube Miller)through somatic embryogenesis Oh,S.D.;W.S.Song
  17. Plant Physiol. v.76 Identification of two methionine transfer RNA genes in the maize mitochondrial genome Parks,T.D.;W.G.dougherty;C.S.Levings 3;D.H.Timothy
  18. Plant Cell, Tissue and Orgen Culture v.12 somatic embryogenesis in cotton (gossypium)L. effects of source of explant and hormone regime Trolinder,N.L.;Goodin
  19. Korean J. Plant Tissue Culture v.18 no.3 Effects of auxins and illumination on growth and nicotine production of calluses induced from Nicotiana glauce leaf explants Yeo,U.D.;K.H.Kim
  20. 감귤원예신서(2nd ed.) 한혜룡;권오균
  21. 감귤원예신서 한혜룡;권오균