Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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In Vitro Propagation of Zanthoxylum piperitum DC. - II. Effect of $NH_4NO_3, KNO_3$ and Casein hydrolysate on Somatic Embryogenesis-

초피나무 (Zanthoxylum piperitum DC) 의 기내증식 - II. $NH_4NO_3, KNO_3$ Casein hydrolysate의 기내 부정배 발생효과 - ( In Vitro Propagation of Zanthoxylum piperitum DC. - II. Effect of NH4NO3 , KNO3 and Casein hydrolysate on Somatic Embryogenesis - )

  • Published : 1995.08.01
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Abstract

Embryogenic callus induces from shoot tip and leaf segment of Zanthoxylum piperitum for producing somatic embryogenesis and plant regeneration were cultured in vitro on Murashige and Tucker's(MT) medium treated with casein hydrolysate $NH_4NO_3$, $KNO_3$ and plant growth regulator. The most effective somatic embryogensis was observed in the medium added by two fold $NH_4NO_3$(3300mg/l)+2. 4-D 0.1mg/l and $KNO_3$(3800mg/l)+2.4-D 0.1mg/l. Also, MT medium supplemented with casein hydrolysate 700mg/l added by 2, 4-D 0.1mg/l were effective in obtainingn somatic embryos from embryogenic callus The effect ofm MT medium supplemented with casein hydrolysate without 2, 4-D was lower than that with (3300mg/l) 2, 4-D for the formation of somatic embryos.

초피(椒皮)나무(Zanthoxylum piperitum DC.)의 embryogenic callus로부터 부정배발생(不定胚發生)에 있어 $NH_4NO_3,\;KNO_3$와 casein hydrolysate의 첨가(添加) 효과(效果)를 알아 보기위하여 본(本) 실험(實驗)을 실시(實施)하였던 바 그 결과(結果)는 다음과 같다. 1. 2,4-D 0.1mg/1가 첨가(添加)된 MT배지(培地)에 $NH_4NO_3$를 3300mg/1 첨가(添加)시킨 배지(培地)에서 부정배발생(不定胚發生)이 가장 양호(良好)하였다. 2. $KNO_3$를 3800mg/1 첨가(添加) 시킨 경우(境遇)에는 MT 기본배지(基本培地)와 2,4-D 0.1mg/1를 첨가(添加)시킨 배지(培地)에서 비교적(比較的) 양호(良好)한 부정배(不定胚)를 발생(發生)시키었다. 3. 2,4-D 0.1mg/1가 첨가(添加)된 MT 배지(培地)에 casein hydrolysate를 700mg/1 첨가(添加)시킨 경우(境遇)에 부정배(不定培) 발생(發生)이 양호(良好)하였으며 2,4-D가 첨가(添加)되지 않는 배지(培地)에서는 부정배(不定培) 발생(發生)이 저조(低調)하였다.

Keywords

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