Antimicrobial Effect of Tea Extracts from Green Tea, Oolong Tea and Black Tea

녹차, 오룡차 및 홍차 추출물의 항균효과

  • 김선봉 (부산수산대학교 식품공학과) ;
  • 여생규 (부산전문대학 식품가공과) ;
  • 안철우 (부산전문대학 식품가공과) ;
  • 김인수 (경상대학교 식품과학과) ;
  • 박영범 (부산수산대학교 식품공학과) ;
  • 박영호 (부산수산대학교 식품공학과)
  • Published : 1995.04.01


Antimicrobial effect of tea extracts from green tea(steamed, roasted), oolong tea and black tea was investigated. Minimal inhibitory concentration(MIC) of tea extraxcts against 9 well known strains of foodborne pathogenic bacteria such as the Gram positive and Gram negative bacteria was determined at 37$^{\circ}C$. Significant antimicrobial activity was observed in the steamed green tea and the roasted green tea of the water-soluble fraciton, and the steamed green tea of the methanol-soluble fraction, and the steamed green tea, roasted green tea and the oolong tea of the crude catechin fraction. The MIC of these extracts against B. subtillis were 700$\mu\textrm{g}$/ml, 500$\mu\textrm{g}$/ml and 120 $\mu\textrm{g}$/ml, respectively. The crude catechin fraction possessed greater antimicrobial activity than did the other fractions. Among tea extracts, extracts of steamed green tea, roasted green tea and oolong tea showed higher antimicrobial activity than them of black tea. The MIC of the crude catechin fraction obtained from tea extracts against Gram-positive bacteria such as M. Iuteus, B. subtillis and S. mutans were 30~50$\mu\textrm{g}$/ml, 120~240$\mu\textrm{g}$/ml and 120~180$\mu\textrm{g}$/ml, and against Gram-negative bacteri such as e. aerogenes and V. parahaemolyticus were 50~60$\mu\textrm{g}$/ml and 60~70$\mu\textrm{g}$/ml in the broth medium, respectively. Especially, the MIC to Streptococcus mutans which has known as a causative bacteria of a decayed tooth were 120$\mu\textrm{g}$/ml, 140$\mu\textrm{g}$/ml, 180$\mu\textrm{g}$/ml and above 1,000$\mu\textrm{g}$/ml in steamed green tea, roasted green tea, oolong tea and black tea, respectively. Tea extracts had strong growth inhibition activity against foodborne pathogenic and dental bacteria.


  1. 한국영양식량학회지 v.21 한약재 추출물의 향균효과 검색 박욱연;장동성;조학래
  2. Agric. Biol. Chem. v.53 Antibacterial substances in Japanese green tea extract against Streptococcus mutans, a carcinogenic bacterium Sakanaka,S.;Mujo,K.;Makoto,T.;Yamamoto,T.
  3. Biosci. Biotech. Biochem. v.56 In vitro effects tea polyphenol intake on human intestinal microflora and metabolism Okubo,T.;Ishihara,N.;Oura,A.;Serit,M.;Kim,M.;Yamamoto,T.;Mitsuoka,T.
  4. 한국영양식량학회지 v.24 녹차, 오룡차 및 홍차 추출물의 돌연변이원성 억제작용 여생규;김인수;안철우;김선봉;박영호
  5. Antibiotics in laboratory medicine(3rd. ed.) Susceptibility testing of antimicrobials in liquid media Lorian,V.
  6. Cultured Diary Products J. v.20 Antibacterial activity associated with Bifidobacterium bifidum Anand,S.K.;Srinirasan,R.A.;Rao,L.K.
  7. Agric. Biol. Chem. v.54 Inhibitory effects of lactic acid bacteria from fermented milk on the mutagenicities of volatile nitrosamines Hosono,A.;Wardojo,R.;Otani,H.
  8. Bifidobacteria Microflora v.7 Bifidobacteria as a residence factor in human beeings Homma,N.
  9. J Food Protection v.51 Inhibition and inactivation of Listeria monocytogenes by sorbic acid EL-Shenawy,M.A.;Martins,E.H.
  10. 日本食品學衛生會誌 v.31 本間ゆかり:ニンニワ押出液の食中毒菌び腐敗細菌に及ぼす抗菌作用 佐藤昭子;寺尾通德
  11. 월간フ-ドケミカル v.5 孟宗竹抽出物の抗菌活性 仁科淳良
  12. 日本食品工業學會誌 v.10 茶葉中の抗酸化成分および抗菌性成分について(第1報),茶藥より 得たフルコ-ル抽出物および水抽出物の抗酸化性および抗菌性について 梶本五郞
  13. 日本農藝化學會誌 v.48 乳酸菌の生育におよぼす綠茶抽出液の阻害作用について 西山隆造;小崎道雄
  14. 日本食品工業學會誌 v.21 綠此抽出液中の抗菌性物質 丹野憲二;野マ村英夫
  15. 日本食品工業學會誌 v.36 茶ボリフェノ-ル類の食中毒細菌に對する細菌活性 原征彦;石上正
  16. 日本食品工業學會誌 v.36 茶飮料類に接種されたA型, B型ボツリスス菌芽胞の動向 原征彦;渡邊眞由美;阪口玄二
  17. 日本食品工業學會誌 v.36 茶ボリフェノ-ル類のボツリスス菌に對する抗菌作用 原征彦;渡邊眞由美
  18. 日本食品工業學會誌 v.36 Streptococcus mutansに對する茶葉カテキンの抗菌作用ついて 川村淳;竹尾忠一
  19. 日本細菌學會誌 v.44 日本茶の抗菌作用および殺菌作用について 戶田眞佐子;大久保幸枝;大西玲子;島村忠勝
  20. 日本農藝化學會誌 v.68 茶葉成分の齒周病原菌に對する抗菌作用 角田隆巳;龍原孝宣;坂根巖
  21. New Food Industry v.29 老化生理と微生物のががわり 水谷武夫