Marchantia polymopha(우산이끼) 현택배양 세포내으 4-methylster이들의 동정

Identification of 4-Demethylsterols from Suspension Cultured Cells of Marchantia polymorpha L.

  • Seong-Ki Kim (Department of Biology, Chung-Ang University)
  • 발행 : 1995.09.01

초록

Five kinds of 4-demethylsterol were isolated from suspension cultured cells of a liverworth, Marchantia polymorpha. Four 4-demethylsterols among them were analyzed by a capillary gas chromatography-massspectrometry and 500 MHz 1H-NMR, and characterized to be avenasterol, 24-methylene-cholesterol, stigmasterol, and campesterol. And the fifth 4-demethylsterol was characterized to be sitosterol by a capillary gas chromatography-mass spectrometry. The concentration of the 4-demethylsterols in the cell decreased in order of avenasterol>stigmasterol>24-methylene-cholesterol>campesterol>sitosterol. When carbon skeleton and the oxidation state was compared with those of brassinosteroids, these 4-demethylsterols may be potent biosynthetic procusors of brassinosteroids in the cells.

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