한국수정란이식학회지 (Journal of Embryo Transfer)
- 제10권3호
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- Pages.209-217
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- 1995
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
2- 및 8- 세포기 생쥐 수정란의 핵이식 및 전기융합법에 의한 복제산자의 생산
Production of cloned Mice by Nuclear Transplantation and Electrofusion Using 2- or 8-Cell Stage Mouse Embryo as Nuclear Donor
초록
The present study was carried out to develop a cloning technology of mouse embryos by nuclear transplantation with electrofusion and to produce cloned offsprings by transfer of reconstituted embryos. A single nucleus from two- and eight-cell embryos was transplanted into the enucleated two-cell embryos by rnicromanipulation. The fusion of nucleus with recipient cytoplasm and the subsequent development of reconstituted embryos in vitro as well as in vivo to term were examined to determine the optimal electrofusion parameters for nuclear transplantation in mouse embryos. The successful enucleation of donor embryos was 84.9 and 83.3% in two- and eight-cell stage, respectively, and the successful injection of nucleus from two- and eight-cell donor embryos into the perivitelline space of enucleated two-cell embryos were 85.1 and 84.7%, respectively. No significant differences were found in enucleation or injection rate between the cell stages of donor embryos. When the blastomeres of intact two-cell mouse embryos were electrofused in 0.3 M mannitol medium(100
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