한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제23권2호
  • /
  • Pages.178-186
  • /
  • 1995
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Streptomyces albus로부터 분리된 Type II Polyketide Synthase 유전자의 염기 서열 및 분석

Nucleotide Sequence and Analysis of the Genes for Type II Polyketide Synthase Isolated from Streptomyes albus

  • 권형진 (명지대학교 생명과학연구소) ;
  • ;
  • 진형종 (명지대학교 생명과학연구소) ;
  • 김수언 (서울대학교 농화학과 및 농업생물 신소재 연구센타) ;
  • 이계준 (서울대학교 분자미생물학 연구센타) ;
  • 서주원 (명지대학교 생명과학연구소)
  • ;
  • Huchinson, C.R. ;
  • ;
  • ;
  • ;
  • 발행 : 1995.05.01
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초록

Streptomyces albus wild type ATCC 21838 produced salinomycin, polyether antibiotic. To clone genes related salinomycin production, a genomic library was screened using actI as a DNA hybridization probe. pWHM 210 was isolated, which contained an approximately 24 kb of insert DNA. A 3.8 kb region in the 24 kb insert DNA was hybridized to actI and the nucleotide sequence of this region was determinied. Two open reading frames found in the same direction were homologous to genes for $\beta$-keto acyl synthase/acyl transferase and chain length determining factor in type II PKS (polyketide synthase). The genes were components of minimal type II PKS genes, highly conserved and showed the strong simiarity to other type II PKS genes known today.

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