Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Nucleotide Sequence and Analysis of the Genes for Type II Polyketide Synthase Isolated from Streptomyes albus

Streptomyces albus로부터 분리된 Type II Polyketide Synthase 유전자의 염기 서열 및 분석

  • ;
  • Huchinson, C.R. ;
  • ;
  • ;
  • ;
  • 권형진 (명지대학교 생명과학연구소) ;
  • ;
  • 진형종 (명지대학교 생명과학연구소) ;
  • 김수언 (서울대학교 농화학과 및 농업생물 신소재 연구센타) ;
  • 이계준 (서울대학교 분자미생물학 연구센타) ;
  • 서주원 (명지대학교 생명과학연구소)
  • Published : 1995.05.01
Download PDF
⟨ Previous Next ⟩

Abstract

Streptomyces albus wild type ATCC 21838 produced salinomycin, polyether antibiotic. To clone genes related salinomycin production, a genomic library was screened using actI as a DNA hybridization probe. pWHM 210 was isolated, which contained an approximately 24 kb of insert DNA. A 3.8 kb region in the 24 kb insert DNA was hybridized to actI and the nucleotide sequence of this region was determinied. Two open reading frames found in the same direction were homologous to genes for $\beta$-keto acyl synthase/acyl transferase and chain length determining factor in type II PKS (polyketide synthase). The genes were components of minimal type II PKS genes, highly conserved and showed the strong simiarity to other type II PKS genes known today.

Keywords

References

  1. Gene v.30 The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein encoding sequence Bibb,M.J.;P.R.Findlay;M.W.Johnson
  2. EMBO J. v.8 Analysis of the nucleotide sequence of the Streptomyces glaucescens tcm I genes provides key information about the enzymology of polyketide antibiotic biosynthesis Bibb,M.J.;B.Sandor;M.Haideh;F.C.John;C.R.Huntchinson
  3. Nature v.348 An unusually large mutifuntional polyketide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea Cortes,J.;S.F.Haydock;G.A.Roberts;D.J.Bevitt;P.F.Leadly
  4. Science v.252 Modular organization of genes required for complex polyketide biosynthesis Donadio,S.;M.J.Staver;J.B.MaAlpine;S.J.Swanson;L.Katz
  5. Gene v.111 Organization of the enzyme domains in the multifunctional polyketide synthase involved in erythromycin formation in Saccharopolyspora eryhraea Donadio,S.;L.Katz
  6. J. Bio. Chem. v.267 Nucleotide sequence and deduced functions of a set of cotranslated genes of Streptomyces coelicolor A3(2) including the polyketide synthase for the antibiotic actinorhodin Fernandez,M.;M.A.;E.Matinez;L.Boto;D.A.Hopwood;F.Malpartida
  7. J.A.C.S. v.116 Engineered biosynthesis of novel polyketide: Dissection of the catalytic specifieity of the act ketoreductase Fu,H.;S.E.Khosla;D.A.Hopwood;C.Khosla
  8. Biochemistry v.33 Engineered biosynthesis of novel polyketide: Stereochemical course of two reaction catalyzed by a polyketide synthase Fu,H.;R.McDaniel;D.A.Hopwood;C.Khosla
  9. Gene v.74 DNA sequence, transcription and deduced function of a gene involved in polyketide antibiotic biosynthesis in Streptomyces coelicolor Hallan,S.E.;F.Malpartida;D.A.Hopwood
  10. Nature v.314 Production of 'hybrid' antibiotics by genetic engineering Hopwood,D.A.;F.Malpartida;H.M.Kieser;H.Ikeda;J.Duncan;I.Fujii;B.A.M.Rudd;H.G.Floss;S.Mura
  11. Annu. Rev. Genet. v.24 Molecular genetics of polyketide and its comparison to fatty acid biosynthesis Hopwood,D.A.;D.H.Sherman
  12. Actinomycetologia v.6 Molecular genetics and biochemistry of typeⅡ polyketide stnthase from the perspective of tetracenomycin C biosynthesis in Streptomyces glaucescens Hutchinson,C.R.;H.Decker;H.Motamedi;B.Shen;R.G.Summer;E.Wendt-Pienkowski;W.L.Wessel
  13. Ann. Rev. Microbiol. v.47 Polyketide synthesis: prospects for hybrid antibiotics Katz,L.;S.Donadio
  14. J. Bacteriol. v.175 Genetic construction and functional analysis of hybrid polyketide synthase containing geterologous acyl carrier proteins Khosla,C.;R.McDaniel;S.E.Khosla;R.Torres;D.A.Sherman;M.J.Bibb;D.A.Hopwood
  15. J. Gen. Microbiol. v.136 Discorvery of an insertion sequence, IS116, from Streptomyces clavuligerus and its relatedness to other transfosable elements from actinomycetes Leskiw,B.K.;M.Mevarech;L.S.Barritt;S.E.Jensen;D.J.Henderson;D.A.Hopwood;C.J.Bruton;K.F.Chater
  16. Nature v.325 Homology between Streptomyces genes coding for synthesis of different polyketide used to clone antibiotic biosynthetic genes Malpatida,F.;S.E.Hallam;H.M.Kieser;H.Motamedi;C.R.Hutchinson;M.J.Butler;D.A.Sugden;M.Warren;C.McKillop;C.R.Bailey;G.O.Humphreys;D.A.Hopwood
  17. Science v.263 Stereospecific acyl transfer on the erythromycin-producing polyketide synthase Marsden,A.F.A.;P.Caffrey;J.F.Aparicio;M.S.Loughran;J.Staunton;P.F.Leadly
  18. J.A.C.S. v.115 Engineered biosynthesis of novel polyketide: Manipulation and analysis of an aromatic polyketide synthase with unproven catalytic specificities McDaniel,R.;S.E.Khosla;D.A.Hopwood;C.Khosla
  19. Science v.262 Engineered biosynthesis of novel polyketide McDaniel,R.;S.E.Khosla;D.A.Hopwood;C.Khosla
  20. JOAC v.116 Engineered biosynthesis of novel polyketide: act Ⅶ and act Ⅳ genes encode aromatase and cyclase enzymes, respectively McDaniel,R.;S.E.Khosla;D.A.Hopwood;C.Khosla
  21. Antimicro. Agents Chemother. v.29 Production of new hybrid antibiotics, mederrhodins A and B. by genetically engineered strain Omura,S.;H.Ikeda;F.Malpatida;H.M.Kieser;D.A.Hopwood
  22. Molecular Cloning: A Laboratory Manual Sambrook,J.;E.F.Fritsch;M.Maniatis
  23. Proc. Natl. Acad. Sci. USA v.74 DNA sequencing with chain termination inhibitor Sanger,F.;S.Nicklen;A.R.Coulson
  24. J. Bacteriol. v.174 Functional replacement of genes for individual polyketide synthase components in Streptomyces coelicolor A3(2) by heterologous genes from a different polyketide pathway Sherman,D.H.;E.S.Kim;M.J.Bibb;D.A.Hopwood
  25. J. Bacteriol. v.174 Nucleotide Sequence of the tcm Ⅱ-tcm Ⅳ Region of the Tetracenomycin C Biosynthetic Gene Clucter of Streptomyces glaucescens and Evidence that the tcmN Gene Encodes a Multifuncthional Cyclase-Dehy-dratase-O-Methyl Transferase Summers,R.G.;E.Wendt-Pienkowski;H.Motamedi;C.R.Hutchinson
  26. Adv. Appl. Microbiol. v.22 Polyether antibiotics: versatile carboxylic acid ionophores produced by Streptomyces Westley,J.W.
  27. Gene v.113 Codon usage in the G+C-rich Streptomyces genome Wright,F.;M.J.Bibb