Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Characterization of Sexual Agglutination and Involvement of Cell-Surface Protein Sexual Cell-Cell Interatrions of Heterobasidiomycetous Yeast

이담자 효모의 세포간 성응집의 특성과 표면단백질의 관련성

  • 정영기 (동의대학교 미생물학과) ;
  • 이태호 (부산대학교 미생물학과) ;
  • 최용락 (동아대학교 농화학과) ;
  • 강원대 (동의대학교 미생물학과)
  • Published : 1995.06.01
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Abstract

When mating type A and a cells of heterobasidiomycetous yeast Rhodosporidium toruloides were mix-cultured, both of the mating type cells have shown strong agglutination. But this agglutination was not detactable when the A and a cell were cultured separately. From reagglutination made just after the result of disassembling the agglutination by sonication, we knew that the agglutination was sexual-agglutination, not simple physical cell agglutination. The sexual agglutination was progressed actively on logarithmic phase and, in addition, progressed faster on mating type a cell treated with rhodotorucine A. These sexual agglutination have been inhibited by several protease such as trypsin, pronase, chymotrpysin and thermolysin and inhibited by 5 mM DTT as well.

Keywords

References

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