Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Opimum Culture Condition of Bullera singularis for Galactooligosaccharide Production

갈락토올리고당 생산 효모 Bullera singularis의 최적 배양조건

  • 신현재 (한국과학기술원 화학공학과) ;
  • 박오진 (한국과학기술원 화학공학과) ;
  • 양지원 (한국과학기술원 화학공학과)
  • Published : 1995.10.01
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Abstract

The cultural conditions of Bullera singularis were optimized for the efficient production of galactooligosaccharide (GOS), Optimum temperature was 25$\circ$C, pH was 6.0, inoculum size was over 5% (v/v), initial lactose concentration was over 5% (w/v). The GOS production increased with microbial growth. Maximum amount of 72% (w/w) GOS was obtained from the optimized medium (5% lactose and 0.75% yeast extract) in 70 hours. Seven types of GOS (3 of dimer, 2 of trimer, 1 of tetramer, and 1 of pentamer) were identified by two-dimensional TLC. A new mechanism of GOS production is proposed based on the metabolism of carbon source.

Keywords

References

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