Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Isolation and Identification of a Bacteriolytic Enzyme-producing Bacterial Strain from Pusan Coastal Sea

해양에서 용균효소를 분비하는 균주의 분리와 동정

  • 진성현 (부산광역시 보건환경연구원) ;
  • 류병호 (경성대학교 식품공학과)
  • Published : 1995.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

In order to produce the bacteriolytic enzyme, bacterial strains capable of excreting a large amount of the enzyme were screened from the coastal sea water samples in Pusan. The bacterial strain SH-1, which showed the highest activity among 43 bacteriolytic enzyme producing bacteria, was finally selected for further studies. The strain SH-1 was an endospore-forming grampositive rod, and the position of spore was paracentral. These morphological characteristics assigned the isolated strain to the morphological group I classified by Gordon. The fatty acid composition of the bacterial stain was analyzed to be consisted of branched chains of iso-Cn and anteiso-Cn. Based on the percent content of the branched chain (93.85%), the isolates could be identified as a species of Bacillus. According to the experimental results of the API system (API 50CHB & API 20E) the strain was identified as Bacillus subtilis. Numerical texonomy, in which 82 major characters were examined using several species of Bacillus as the standard bacteria, indicated that the strain SH-1 showed 90% similarity to Bacillus subtilis. Thus, the isolated strain SH-1 could be identified as Bacillus subtilis.

Keywords

References

  1. Bacteriol. Rev. v.32 Use of bacteriolytic enzymes in determination of wall structure and their role in cell metabolism Ghuysen,J.M.
  2. Nippon Suisan Gakkaishi v.54 Distribution and generic composiotion of lytic enzyme-producing bacteria in cosastal and oceanic bottom sediments Sugahara,I.;T.Kimura;K.Hayashi;I.Nakajima
  3. Nippon Suisan Gakkaishi v.56 Lytic enzyme producing bactria in marine bottom sediments. Sugahara,I.;T.Kimura;Y.Nakagawa;H.Kojima;M.Usuda;M.Tank
  4. Nippon Suisan Gakkaishi v.54 Effect of Temperature and pressure on the lytic and autolytic activities of Bacillus sp. V37 isolated from coastal regions Sugahara,I.;L.R.Berger;T.Kimura;K.Hayashi
  5. J. Bacterol. v.90 structure and composition of Bacillus anthracis capsule Avakyan,A.A.;L.N.Levina;I.B.Pavlova
  6. Fed-proc v.26 Cytology of Bacillus anthrcis Gerhardt,P.
  7. J. Bactriol. v.85 Classification of microorganism by analysis of chemical composition. I. Flasability of utilizing gaschromatography Abel,K.;H.deShmertzing;J.I.Peterson
  8. Hewlett-Packard Application Note Bacteria identification by gas chromatography of whole cell fatty acid Miller,L.;T.Berger
  9. Biochemical tests for Identification of Medical Bacteria,2nd ed. MacFaddin,J.F.
  10. Manual for the identification of medical bacteria,2nd ed. Cowan,S.T.;Steel,K.J.
  11. Bergey's manual of systematic bactriology v.2 Sneath,P.H.
  12. The genus Bacillus handbook No.427 U.S.Department of Agriculture Gordon,R.E.;W.C.Haynes;C.H.N.Pang
  13. Classification and identification of the genus Bacillus using API tests, In the Aerobic endospore-forming bacteria: Classification and identification Logan,N.A.;R.C.W.Berkeley(ed.);M.Goodfellow(ed.)
  14. J. Gen. Microbiol. v.130 Identifocaiton of Bacillus strains using the API system Logan,N.A.;R.C.W.Berkeley
  15. Diagnostics Microbiology Keneman,E.W.;S.D.Allen;V.R.Dowell;W.H.Jonda;H.M.Sommers;W.C.Winn
  16. Manual of clinical microbiology Turnburn,P.C.B.;J.M.Kramer
  17. The genus Bacillus: A numerical analysis. In the aerobic endospore-forming bacteria: Classification and identification Priest,F.G.;M.Goodfellow;C.Todd;R.C.W.Berkely(ed.);M.Goodfellow(ed.)
  18. J. Gen. Microbilo. v.134 A Numerical Classification of the Genus Bacillus Priest,F.G.M.;Goodfellow;C.Todd
  19. International J. Systematic Bacteriol. v.30 Characterization of Bacillus subtilis, Bacillus pumilus, Bacillus licheniformis and Bacillus amyloli quefaciens by pyrolysis gas-liquid chromatiography, deoxyribonucelic acid-deoxyribonucleic acid hybidization,biochemical tests and API system O'Donnell,A.G.;J.R.Norris;R.C.W.Berkeley;D.Claus;T.Kaneko;N.A.Logan;R.Nozaki
  20. Chemical methods in bacteria systematics Goodfellow,M.;D.E.Minnikin
  21. J. Bacteriol. v.93 Fatty acid in the genus Bacillus. I. Iso-and anteisofatty acid as characteristic constituents of lipid in 10 Species. Kaneda,T.
  22. Bacteriological Reviews v.41 Fatty acid of the genus Bacillus. An example of branched-chain prefernece. Kaneda,T.
  23. Appl. Environ. Microbiol. v.35 Determination of fatty acid composition of Bacillus cereus and related bacteria: A rapid gas chromatographic method using a glass capillary column Niskaman,A.;T.Kiutamo;S.Raisanen;M.raevuori
  24. Numerical Toxonomy Sneath,peter H.A.;Robert R. Sokal
  25. Modern Bacterial Taxonomy Austin,B.;F.Priest
  26. Statistical Ecology Ludwing, Johan A.;James F. Reynolds
  27. Appl. Microbiol. v.10 Heat activation and heat-induced dormancy of Bacillus stearothermophilus spores. Finley,N.;M.L.Fields
  28. B. Review v.41 Fatty acid of the genus Bacillus. An example of branched-chain perfernce Kaneda,T.
  29. J. Gen. Microbiol. v.132 Characterization of Flavobacterium species analysis of volatile fatty acid production Rasoamananjaka,D.;F.Peladan;J.C.Turlot;H.Monteil;L.Richard
  30. J. Biol. Chem. v.238 Biosynthesis of branched chain fatty acids. I. Isolation and identification of fatty acids from Bacillus subtilis(ATCC 7059) Kaneda,T.
  31. Biochemistry v.7 Biosynthesis of long-chain hydrocarbons.Ⅱ, Stdudies on the biosynthetic pathway in tobacco Kaneda,T.
  32. J. Bacteriol. v.98 Fatty acids in Bacillus larvae, Bacillus lentimorbus, and Bacillus popilliae Kaneda,T.
  33. Can. J. Biochem. Physiol. v.40 Lipids of Halobacterium cutirubrum. Sehgal,S.N.;M.Kates;N.E.Gibbons