Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Cell Distribution and Shear Effect on Mammalian Cell Growth in Macroporous Gelatin Microcarriers

다공성 젤라틴 미립담체를 이용한 동물세포의 배양시 담체 내외부의 세포 분포 및 전단응력의 영향

  • 임현수 (한국과학기술원 생물공학과) ;
  • 김정회 (한국과학기술원 생물공학과)
  • Published : 1995.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

Immobilization of anchorage-dependent animal cells was investigated using macroporous gelatin microcarriers developed in our laboratory. For the observation of the distribution of cells in macroporous beads, Vero-6 cells and CHO cells were cuttured and their distribution in macroporous beads was observed using a confocal microscope. In results, the final concentration of Vero-6 cells and CHO cells on macroporous beads was 2-3 times higher than that on commercial solid microcarriers (Cytodex-3). Also, macroporous microcarriers could hold cells in their macropores. Consequently, the pores protected cells against hydrodynamic shear. Based on Kolmogorov eddy length scale, the smaller eddies (80 $\mu $m) showed the detrimental effect on cells in macroporous beads as compared with 160 $\mu $m of eddies in conventional solid microcarriers.

Keywords

References

  1. Tissue culture: Methods and application Van Wezel,A.L.(ed.)
  2. Biotech. Bioeng. v.27 A mechanistic analysis of the inoculum requirement for the cultivation of mammalian cells on microcarriers Hu,W.S.;J.Meier;D,I,C,Wang
  3. Biotech. Bioeng. v.27 Sereal propagation of mammalian cells on microcarriers Hu,W.S.;D.J.Giard;D.I.C.Wang
  4. Biotech. Bioeng. v.30 Selection of microcarrier diameter for the cultivation of mammalian cells on microcarriers Hu,W.S.;D.I.C.Wang
  5. Proc. APBioChEC'90 Developement of macroporous gelation microcarrier and its characterization for anchorage-dependent animal cell culture Lim,H.S.;J.H.Choi;J.H.Kim
  6. Large scale cell culture technology Continuous cell culture with fluidized sponge beads Dean,R.C.;S.B.Karkare;P.G.Philips;N.G.Ray;P.W.Runstadler
  7. Cytotechnology v.2 Initial culture conditions affect the sensitivity of HepG2 cells to excessive mechanical agitation KIm,J.H.;W.S.Hu
  8. Biotech. Bioeng. v.32 Physical mechanisms of cell damage in microcarrier cell culture bioreacters Cherry,R.S.;E.T.Papoutasakis
  9. Biotech. Bioeng. v.36 Damage mechanisms of suspended animal cells in agitated bioreactors with and without bubble entrainment Kunas,K.T.;E.T.Papoutsakis