Taxol Production in Taxus Cell Cultures: Effects of Various Elicitors

주목세포배양에 의한 Taxol 생산: 여러 가지 Elicitor가 미치는 영향

The effects of various elicitors, metabolic inhibitors and growth regulators on the production of diterpenoid anticancer agent taxol were investigated in cell suspension cultures of Taxus brevifolia. Cell cultures of T. brevifolia were treated by 5 kinds of biotic elicitors, 5 kinds of abiotic elicitors, 2 kinds of metabolic inhibitors and 8 kinds of growth regulators at the end of exponential growth phase. Among those treatments, chlorocholine chloride-an inhibitor of plant steroid metabolism-increased the taxol production most significantly. From a series of optimization studies, it was found that the addition of 1mM of chlorocholine chloride at the 9th day of culture was the best for taxol production. Taxol yield under this condition was 0.72mg/$\ell$.

Taxus brevifolia 현탁세포배양에 서 항암제 taxol의 생산을 향상시키기 위해 5종류의 biotic elicitor 와 5종류의 abiotic elicitor, 2종류의 대사억제 및 8종류의 생장조절제를 세포배양 중에 첨가하여 효과 가 았는 물질을 선별하였다. T. brevifo/ia 현탁세포 배양의 대수증식기 말기인 10일째에 각각의 물질을 첨가하여 배양액 내의 taxol의 함량을 측정한 결과 steroid계 억제제 인 chlorocholine chloride를 처리 하였을 때 taxol의 생성이 현저히 증가되었다. Chloro choline chloride의 처리시기 및 처리농도를 다르게 하여 최적조건을 찾은 결과 9일째에 1mM을 첨가했 을 경우 taxol 생성이 가장 좋았다.