Journal of Microbiology

  • 제33권3호
  • /
  • Pages.196-200
  • /
  • 1995
  • /
  • 1225-8873(pISSN)
  • /
  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Heat Inducible Expression of the CDC70 Gene Under the Control of Heat Shock Element in Saccharomyces Cerevisiae

  • 발행 : 1995.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In order to express the CDC70 gene of Saccharomyces cerevisiae by heat shock, we have designed heat inducibe hybrid promoters using the Drosophila melanogaster heat shock elements (HSEs). A 220 bp-long upstream fragment of the D. melanogaster hsp70 gene comprised of four HSEs was placed upstream of the putative proximal TATA box of the CDC70 gene. Hybrid promoters containing different fusion joints were tested for their ability to drive the CDC70 gene expression by heat shock. The results showed that the HSEs of D. melanogaster conferred the heat-induced CDC70 gene expression, but the heat inducibility was much lower than that in D. melanogaster.

키워드

참고문헌

  1. Cell v.45 Heat shock regulatory elements function as inducible enhancer in the Xenopus hsp gene and when linked to a heterologous promoter Bienz,M.;H.R.B.Pelham
  2. Proc. Natl. Acad. Sci. U.S.A. v.82 Specific protein binding to far upstream activating sequences in polymerase Ⅱ promoters Bram,R.;R.Kornberg
  3. Cell v.40 Specific DNA binding of GAL4, a positive regulatory protein of yeast Giniger,E.;S.M.Varnum;M.Ptashne
  4. Cell v.36 Yeast promoters: Positive and negative elements Guarente,L.
  5. Proc. Natl. Acad. Sci. U.S.A. v.78 Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae Guarente,L.;M.Ptashne
  6. Proc. Natl. Acad. Sci. U.S.A. v.75 Transformation of yeast Hinnen,A.;J.Hichs;J.Fink
  7. Cell v.46 Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of yeast Hope,I.A.;K.Struhl
  8. Cell v.44 Translational and transcriptional control elements in the untranslated leader of the heat-shock gene hsp22 Hultmark,D.;R.Klemenz;W.J.Gehring
  9. Ph. D Dissertation, Seoul National University Studies on the start genes in the control of cell cycle and mating processes in Saccharomyces cerevisiae Jahng,K.Y.
  10. Mol. Cell. Biol. v.8 Mutations in a gene encoding the alpha subunit of a Saccharomyces cerevisiae G protein indicate a role in mating pheromone signaling Jahng,K.Y.;J.Ferguson;S.I.Reed
  11. Science v.231 Separation of DNA binding from the transcription-activating function of eukaryotic regulatory protein Keegan,L.;G.Gill;M.Ptashne
  12. EMBO J. v.4 Selective translation of heat shock mRNA in Drosophila melanogaster depends on sequence information in the leader Klemenz,R.;D.Hultmark;W.J.Gehring
  13. Mol. Cell. Biol. v.8 Sex-specific control of Drosophila melanogaster yolk protein I gene expression is limited to transcription Kraus,K.;Y.Lee;J.T.Lis;M.Wolfner
  14. Ann. Rev. Biochem. v.55 The heat shock response Lindquist,S.
  15. Molecular cloning: A laboratory manual Maniatis,T.;E.F.Fritsch;J.Sambrook
  16. Cell v.46 Genetic complementation of a glucocorticoid receptor deficiency by expression of cloned receptor cDNA Miesfeld,R.;S.Rusconi;S.Okret;A.C.Wikstrom;J.A.Gustafsson;K.R.Yamamoto
  17. EMBO J. v.1 Regulation of heat-shock gene: A DNA sequence upstream of Drosophila hsp70 gene is essential for their induction in monkey cells Mirault,M.E.;R.Southgate;E.Delwart
  18. Cell v.36 A Drosophila RNA polymerase Ⅱ transcription factor contains a promoter-region-specific DNA binding activity Parker,C.S.;J.Topol
  19. Cell v.30 Regulatory upstream promoter element in the Drosophila hsp70 heat-shock gene Pelham,H.R.B.
  20. EMBO J. v.1 A synthetic heat-shock promoter element confers heat-inducibility on the herpes simplex virus thymidine kinase gene Phlham,H.B.R.;M.Bienz
  21. Cell v.37 Structural and functional analysis of the human metallothionein-IA gene: Differential induction by metal ions and glucocorticoids Richards,R.I.;A.Heguy;M.Karin
  22. Cell v.43 Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region Sawadogo,M.;R.G.Roeder
  23. Cell v.40 Determinants of heat-shock induced chromosome puffing Simon,J.A.;C.A.Sutton;R.B.Lobell;R.L.Glaser;J.T.Lis
  24. Cell v.48 The Saccharomyces and Drosophila heat-shock transcription factors are identical in size and DNA binding properties Wiederrecht,G.;D.J.Shuey;W.A.Kibbe;C.S.Parker
  25. Nature v.309 Two protein-binding sites in chromatin implicated in the activation of heat-shock genes Wu,C.
  26. Science v.239 Germline transformation used to define key features of heat-shock response elements Xiao,H.;J.T.Lis