Toxicological Research

  • 제11권2호
  • /
  • Pages.315-327
  • /
  • 1995
  • /
  • 1976-8257(pISSN)
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  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
권호 표지

마약류 및 산업환경화학물질에 의한 GFAP의 신경독성표지물질화에 관한 유용성

The Neurotoxicological Alterations Induced by Narcotic Drugs and Industrial Chemicals in the Rat are Associated with Quantitative Changes in Glial Fibrillary Acidic Protein

  • 조대현 (국립보건안전연구원 독성부) ;
  • 정용 (국립보건안전연구원 독성부) ;
  • 김준규 (국립보건안전연구원 독성부) ;
  • 이봉훈 (국립보건안전연구원 독성부) ;
  • 황세진 (국립보건안전연구원 독성부) ;
  • 이원용 (국립보건안전연구원 독성부) ;
  • 김정구 (국립보건안전연구원 독성부) ;
  • 조태순 (성균관대학교 약학대학) ;
  • 김진석 (건국대학교 수의과대학) ;
  • 문화회 (국립보건안전연구원 독성부)
  • Cho, Dae-Hyun (Department of Toxicology, National Institute of Safety Research) ;
  • Jeong, Yong (Department of Toxicology, National Institute of Safety Research) ;
  • Kim, Jun-Gyou (Department of Toxicology, National Institute of Safety Research) ;
  • Lee, Bong-Hun (Department of Toxicology, National Institute of Safety Research) ;
  • Hwang, Se-Jin (Department of Toxicology, National Institute of Safety Research) ;
  • Lee, Won-Yong (Department of Toxicology, National Institute of Safety Research) ;
  • Kim, Jeong-Goo (Department of Toxicology, National Institute of Safety Research) ;
  • Cho, Tai-Soon (College of Pharmacy, Sung Kyun Kwan University) ;
  • Kim, Jin-Suk (College of Veterinary Medicine, Kon-Kuk University) ;
  • Moon, Hwa-Hwey (Department of Toxicology, National Institute of Safety Research)
  • 발행 : 1995.12.01
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초록

Diverse neurotoxic insults result in proliferation and hypertrophy of astrocytes, a subtype of glia in central nervous system. The hallmark of this response, often terms "reactive gliosis", is the enhanced expression of the major intermediate filament protein of castrocytes, glial fibrillary acidic protein (GFAP). These changes in the astrocytes suggest that GFAP may be a useful biochemical indicator of neurotoxicity. To investigate this possibility, we administered intra-peritoneally prototype nerotoxicants, metharnphetamine (MAP, 5 mg/kg), cocaine (30 mg/kg), N-buthyl benzenesulfonamide (NBBS, 300 mg/kg) and trimethytin (TMT, 8 mg/kg) to Wistar Rats and then assessed the effects of these agents on content of GFAP, which were determined by Sandwish ELISA and evaluated with neurotoxic symptoms, and quantitative changes of imrnunoreactivity of GFAP by light microscopic image analysis in specific regions. We found that assay of GFAP revealed time- and region-dependant patterns of neurotoxicity. The GFAP immunoreactivity of rat brain was increased in substantia nigra and hippocampus by MAP, NBBS and TMT; in roedial septal nucleus and nucleus accurnbens, it was also increased by RrBBS. Sandwich ELISA showed that GFAP levels of cerebrum in all groups on days 3 and 7 and that of brainstem(including cerebellum) in MAP, NBBS groups on day 1 and 3 were increased. A review of the background, design and results of these experiments are presented in this paper. Our findings indicate that GFAP is a sensitive and specific biomarker of neurotoxicity.otoxicity.

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