Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Nephrotoxicity Assessment of Cephaloridine using Rat Renal Proximal Tubule Suspension

랫트의 신장 근위곡세뇨관 현탁액을 이용한 Cephaloridine의 신장독성 평가

  • 홍충만 (국립보건안전연구원 병리부) ;
  • 장동덕 (국립보건안전연구원 병리부) ;
  • 신동환 (국립보건안전연구원 병리부) ;
  • 최진영 (국립보건안전연구원 병리부) ;
  • 조재천 (국립보건안전연구원 병리부) ;
  • 이문한 (서울대학교 수의과대학)
  • Published : 1995.06.01
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Abstract

Rat renal proximal tubule suspension was prepared from adult male Sprague Dawley rat (250-300g) by mechanical (non-enzymatical) method and evaluated as a pontential model for mechanistic studies and early screening of nephrotoxicity, using anionic antibiotics (cephaloridine). Cephaloridine (CPL) produced an increase in LDH release into media. This release results from decrease a proximal tubule cell viability and subsequently increase the permeability of cell viability and subsequently increase the permeability of cell membrane. Since loss of intracellular potassium and ATP into media is the sign of disruption of cell membrane, especially basolateral membrane (BLM), CPL induced proximal tubule cell compromise also appear be associated with BLM, maybe $Na^+-K^+$ ATPase. Also seen was significant depression in brush border membrane (BBM) ALP activity and no significantly increase in BBM GGT activities. The inhibition of typical anion, PAH accumulation (especially, CPL 5 mM) and cation, TEA (especially, 4hours incubation) were seen dose dependently. This is because of CPL accumulation in renal proximal tubule and increase of cytotoxicity.

Keywords

References

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