Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
권호 표지

Application of X-Y Dissociation of Mice as the in vivo Assaying System for Environmental Mutagens

Mouse 제 1정모세포에서의 X-Y 염색체 조기 분리;in vivo 환경성 변이원 검출계로서의 응용 가능성

  • 최영현 (부산대학교 자연과학대학 생물학과) ;
  • 권용원 (부산대학교 자연과학대학 생물학과) ;
  • 최병태 (부산대학교 사범대학 생물교육과) ;
  • 조운복 (부산대학교 사범대학 생물교육과) ;
  • 이원호 (부산대학교 자연과학대학 생물학과)
  • Published : 1995.06.01
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Abstract

The present experiment was carried out to investigate whether X and Y chromosome dissociation in the primary spermatocytes of mice can be used as an in vivo assaying system that detect environmental mutagens. For this purpose, alkylating agents (EMS, MMS and MMC), which are strong mutagens, were administered to ICR male mice 12-15 weeks old. The mean frequencies of previously dissociated X-Y chromosomes and autosomes of the control group were 7.34-7.45% and 0.92-1.04%, respectively. The frequencies of X-Y dissociation in the mutagen-treated groups with 10.0 mM EMS and 5.0 mM MMS were about 3.3-4.6 times higher than that in the control group, but there were no significant differences in dissociation of autosomes in both the control and the mutagen-treated groups. These results suggest that X-Y dissociation in the primary spermatocytes of mice can be used as an in vivo short-term assaying system for environmental mutagens.

Keywords

References

  1. Mutat. Res. v.31 Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test Ames,B.N.;McCann,J.;Yamasaki,E.
  2. Cytogenet. Cell Genet. v.12 X-Y chromosome dissociation and sterility in the mouse Beechey,C.V.
  3. Kor. J. Zoology v.37 Analysis of chiasma, univalents and X-Y dissociation in Korean wild mice(Mus musculus subspecies) Choi,Y.H.;Kwon,Y.W.;Lee,W.H.
  4. Kor. J. Lab. Ani. Sci. v.10 Chromosome dissociation and histological study of testis in the sterile male ICR mice Choi.Y.H.;Choi.B.T.;Jo,U.B.;Lee,W.H.
  5. Kor. J. Gerontol. v.4 Study on age-related meiotic chromosomeal and histological changes in the testis of Senescence accelerated mice Choi.Y.H.;Choi.B.T.;Chung,H.Y.;Jo,U.B.;Lee,W.H.
  6. Nature v.300 Meiotic crossing-over between the X and Y chromosomes of male mice carrying the sex-reversing (Sxr) factor Evans,E.P.;Burtenshaw,M.D.
  7. Z. mikr.-anat. Forsch. v.12 Chromosomen analyse der reziproken Bastarde zwischen Pygaera pigra und P. curtula sowie ihrer Ruckkreuzungsbastarde Federley,H.
  8. Cytogenet. Cell Genet. v.29 A search for a gebetic basis for gonosomal univalency in mice Gollapudi,B.B.;Kamra,O.P.;Blecher,S.R.
  9. Natrue v.218 Chiasma frequency and maternal age in mammals Henderson,S.A.;Edwards,R.G.
  10. Jpn. J. Genet. v.65 The sex chromosome association (Scx) gene is loscted on the X-chromosome in mice Imai,H.T.;Wada,M.Y.;Moriwaki,K.
  11. Cytogenet. Cell Genet. v.2 High frequency of X-Y chromosome dissociation in primary spermatocytes of F1 hybrids between Japanese wild mice (Mus musculus molossinus) and inbred laboratory mice Imai,H.T.;Matsuda,Y.;Shiroishi,T.;Moriwaki,K.
  12. Chromosoma v.85 A re-examination of chiasma terminalization and chiasma frequency in male mice Imai,H.T.;Moriwaki,K.
  13. Cytogenet. Cell genet. v.23 Analysis of diplotene chiasma frequencies in mouse oocytes and spermatocytes in relation to ageing and sexual dimorphism Jagiello,G.;Fang,J.S.
  14. Jpn. J. Genet. v.44 Male meiosis in flesh-flies of the genus Parasarcophaga (Sarcophagidae: Diptera) Kaul,D.;Gaur,P.;Tewari,R.R.
  15. Can. J. Genet. Cytol. v.13 Spontaneous meiotic chromosome abnormalities in male mice(Mus musculus) Lin,C.C.;Tsuchida,W.S.;Morris,S.A.
  16. Cytogenet. Cell Genet. v.12 Chiasma and univalent frequencies in aging female mice Luthardt,F.W.;Palmer,C.G.;Yu,P.L.
  17. Chromosoma v.97 Univalent sex chromosomes in spermatocytes of Sxr-carrying mice Mahadevaiah,S.;Setterfield,L.A.;Mittwoch,U.
  18. J. Heredity v.70 Mutagenicity studies with 5-thio-D-glu-cose Majumdar,S.K.;Buchanman,B.;Feinstein,S.
  19. Cytogenet, Cell Genet. v.34 X-Y chromosome dissociation in wild derived Mus musculus molossinus subspecies, laboratory mice, and their F1 hybrids Matsuda,Y.;Imai,H.T.;Moriwaki,K.;Kondo,K.;Bonhomme,E.
  20. Cytogenet. Cell Genet. v.35 Modes of inheritance of X-Y dissociation in intersubspecies hybrids between BALB/c mice and Mus musculus molossinus Matsuda,Y.;Imai,H.T.;Moriwaki,K.;Kondo,K.
  21. Proc. Natl. Acad. Sci. (USA) v.72 Detection of carcinogens as mutagens in Salmonella/microsome test: Assay of 300 chemicals McCann,J.;Choi,E.;Yamazaki,E.;Ames,B.N.
  22. Directed Mutagenesis: a practical approach McPherson,M.J.
  23. Cytogenet. Cell Genet. v.13 Sex-chromosome pairing and male fertility Miklos,G.L.G.
  24. Cytotogenet. Cell. Genet. v.12 Spontaneous univalence at male meiosis in the mouse Purnell,D.J.
  25. Cytogenet. Cell. Genet. v.19 Nonpairing of the X and Y chromosomes in the spermatocytes of BDF1 mice Rapp.M.;Therman,E.;Denniston,C.
  26. Chromosoma v.29 The spatial relationship of X and Y chromosomes during meiotic prophase in mouse spermatocytes Solari,A.J.
  27. Chromosoma v.64 The effects of ageing on the meiotic chromosomes of male female mice Speed,R.M.
  28. Cytogenet. Cell Genet. v.35 Absence of correlation between univalent formation and meiotic nondisjunction in aged female Chinese hamster Sugawara,S.;Mikamo,K.
  29. Cancer v.49 Mutagens, carcinogens, and tumor promoters in our daily foods Sugimura,T.
  30. General Genetics Synder,L.A.;Freifflder,D.;Hartl,D.L.
  31. Mutat. Res. v.37 A correlative study on the genetic damage induced by chemical mutagens in bone marrow and spermatogonia of mice, Ⅰ. CNU-ethanol Tates,A.D.;Natarajan,A.T.
  32. Mutagenicity testing: a practical approach Ventitt,S.;Party,J.M.
  33. The Predictive Value of Short-term Screening Tests in Carcinogens Some current perspectives of the application of Drosophila in the evaluation of carcinogens Vogel,E.W.;Bljileven,W.G.H.;Klapwijk,P.M.;Zjilstra,J.A.;Williams,G.M.(et al.)(eds.)
  34. Chemical Mutagens v.10 In vivo mutagenicity tests using somatic cells of Drosophila melanogaster Wurgler,F.E.;Vogel,E.W.;de Serres,F.J.(ed.)