The Korean Journal of Zoology (한국동물학회지)

The Korean Society for Integrative Biology (한국통합생물학회)
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Endogenous Gene Expression of p53 and Regulatory Subunits of Cyclic AMP-dependent Protein Kinase in Ovarian Cancer Cells

난소암 세포주에서 p53과 Cyclic AMP-dependent Protein Kinase의 Regulatory Subunit 유전자들의 발현에 관한 연구

  • Jin Seo (Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University) ;
  • Park, Woonmee (Samsung Biomedical Research Institute) ;
  • Hwang, Eun-Seong (Samsung Biomedical Research Institute) ;
  • Lee, Je-Ho (Samsung Biomedical Research Institute) ;
  • Hong, Seung-Hwan (Institute for Molecular Biology and Genetics and Department of Molecular Biology, Seoul National University)
  • Published : 1995.04.01
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Abstract

In an effort to develop a new therapeutic strategy for human gene therapy of solid ovarian tumor, we studied the expression of the p53 tumor suppressor Sene as well as regulatory subunits of cyclic AMP (cAMP)-dependent protein kinase in human ovarian carcinoma cells. Four cell lines (2774, Caov-3, SK-OV-3 and OVCAR-3) were selected for the analyses. The p53 transcript and protein were detected only in the 2774 cell line by Northern and Western Bnalysis. In the relatively fast growing cell line, SK-OV-3, the %rope 1 a regulstorv subunit (RIA of CAMP-dependent protein kinase was the highest among the four cell lines. The expression level of $RII\beta$ protein was low in the four cell lines examined. These results maw point to a direction to select the target gene(sl to be employed for gene therapy to control the ovarian cancer.

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