Korean Journal of Animal Reproduction (한국가축번식학회지)
- Volume 19 Issue 2
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- Pages.89-93
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- 1995
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- 1226-5284(pISSN)
Transfer and Expression of E. coli LacZ Gene in Boving Embryos by Co-culturing with Retrovirus Vector-Producing Cells
Retrovirus Vector를 생산하는 세포와 공동배양된 소 수정란의 E. coli LacZ 유전자 전이와 발현
Abstract
In this study was demonstrate that retrovirus-mediated gene transfer is one of the promising alternatives to the conventional pronuclear DNA microinjection approach, especially in transferring the exogenous genes into the boving embryos. By co-culturing of zona of zona-free one-cell stage embryos with the retrovirus-producing cells for 24 hours followed by 6 days of culture in virus-free medium, we could get morulae and blastocysts expressing the E. coli LacZ genes which were transferred by our retrovirus vector. The results obtained in this study are summarized as follows : 1. Addition of 5
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