대한의용생체공학회:의공학회지 (Journal of Biomedical Engineering Research)

  • 제16권1호
  • /
  • Pages.1-8
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  • 1995
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  • 1229-0807(pISSN)
  • /
  • 2288-9396(eISSN)
대한의용생체공학회 (The Korean Society of Medical and Biological Engineering)
권호 표지

소구경 폴리우레탄 인공혈관의 개발을 위한 세포외기질위의 혈관내피세포 배양

Endothelial Cell Seeding Onto the Extracellular Matrix of Fibroblasts for the Developement of Small Diameter Polyurethane Vessel

  • 박동국 (단국대학교 의과대학 일반외과학교실) ;
  • 이윤신 (서울대학교 의과대학 의공학연구소, 순천향대학 부속병원 성형외과학교실, 서울대학교 외과대학 병리학교실)
  • 발행 : 1995.03.01
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초록

A variety of experiments of endothelial cell seeding onto artificial vessels have been performed. To improve endothelialization, one or two components of the extracellular matrix (ECM) have been used as an underlying matrix. In this study, the whole ECM excreted from fibroblasts was used as an underlying matrix. Fetal human fibroblasts were cultured on a polyurethane (PU) sheet. After a conflu; ence was attained, the cytoskeleton and the nuclei of the fibroblast were destroyed using Triton-X. Mitomycin, or irradiation. Omental microvascular endothelial cells from adult human were seeded onto various substrates. After 12 days in culture, the cells were counted. It was observed that the ECM treated by irradiation had the highest cell number. In addition, the cells on this substrate exhibited the most typical endothelial cell morphology. For preliminary animal experiments the PU vessels (inner diameter, 1.5mm) coated with ECM were implanted in the infrarena] abdominal aorta of rat. After the vessels had been implanted for 5 weeks, it was found that the surface of the PU vessels was completely covered with endothelia] cells. In conclusion, we can state that the fibroblast-derived whole ECM makes a better underlying substrate for the endothelialization of small diameter artificial vessels.

키워드

참고문헌

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