약학회지 (YAKHAK HOEJI)

  • 제39권6호
  • /
  • Pages.654-660
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  • 1995
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  • 0377-9556(pISSN)
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  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

알록산 유도 당뇨흰쥐의 폐에서 황산화계의 변화

The Changes of Antioxidant Enzymes in the Lung of Alloxan-induced Diabetic Rats

  • 발행 : 1995.12.01
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초록

The present study was attempted to investigate the mechanism of oxidative cellular injuries which occur in diabetic rats by determining changes of antioxidant enzymes activity in the lung of alloxan-induced diabetic rats, the contents of glutathione in the lung, liver, blood samples, and ${\gamma}$-glutamylcysteine synthetase activities in the liver. Superoxide dismutase activities (SOD), including Cu, Zn-SOD and Mn-SOD, decreased in the lung of diabetic rats compared with those of normal control rats. However, activities of catalase and glutathione peroxidase(GPX) activities were not affected in the lung of diabetic rats. In diabetic rats, glutathione contents in the lung, liver, and blood samples, as well as the activities of ${\gamma}$-glutamylcysteine synthetase in the livers which is known to be the key enzyme of glutatione biosynthesis, decreased significantly. From these experimental results, it is thought that the decrease in SOD activities in the lung, glutathione contents and ${\gamma}$-glutamylcysteine synthetase activities in some tissues in alloxan-induced diabetic rats may be the crucial cause of vullnerability to oxidative cellular injuries.

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참고문헌

  1. Arch. Biochem. Biophys. v.247 Biological effects of the superoxide radical Fridovich,I.
  2. CRC handbook of free radical and antioxidants in biomedicine v.Ⅰ Historical introduction to free radical and antioxidant biomedical research Miquel,J.;Miquel,J(ed.);Quintaniha,A.T.(ed.);Weber,H.(ed.)
  3. CRC handbook of free radical and antioxidants in biomedicine v.Ⅰ Free radicals and human disease Proctor,P.H.;Miquel,J(ed.);Quintaniha,A.T.(ed.);Weber,H.(ed.)
  4. CRC handbook of free radical and antioxidants in biomedicine v.Ⅰ Free radical-induced lipoperoxidation and aging Lippman,R.D.;Miquel,J(ed.);Quintaniha,A.T.(ed.);Weber,H.(ed.)
  5. Methods enzymolgy v.186 Roles of free radicals and catalytic metal ions in human disease: An overview Halliwell,B.;Gutterridge,J.M.C.;Fleischer,S.(ed.);Packer,L.(ed.)
  6. Diabetes v.30 The inhibition of islet superoxide dismutase by diabetogenic drugs Crouch,R.K.;Gandy,S.E.;Kimsey,G.;Galbraith,R.A.;Galbraith,G.M.P.;Buse,M.G.
  7. Diabetologia v.31 Alloxan:history and mechanism of action Lensen,S.;Panten,U.
  8. Oxidative stress Endogenous antioxidant defences in human blood plasma Stocker,R.;Frei,B.;Sies,H.(ed.)
  9. Molecular and Cellular Biochemistry v.84 Antioxidant enzyme alterations in experimental and clinical diabetics Godin,D.V.;Wohaieb,S.A.;Garnett,M.E.;Goumeniouk,A.D.
  10. Horm. Metab. Res. v.14 The effect of diabetes on the activities of the peroxide metabolism enzymes Matkovics,B.;Varga,S.I.;Szabo,L.;Witas,H.
  11. Endocrinology v.111 Superoxide dismutase activity in the intestinal mucosa of the streptozotocin-diabetic rat Loven,D.P.;Schedl,H.P.;Oberley,L.W.;Wilson,H.D.;Bruch,L.;Niehaus,C.N.
  12. Diabetes v.35 Effect of insulin and oral glutathione on glutathione levels and superoxide dismutase activities in organs of rats with streptozotocin-induced diabetes Loven,D.;Schedl,H.;Wilson,H.;Daabees,T.T.;Stegink,L.D.;Diekus,M.;Oberley,L.
  13. Acta Endocrinologica v.102 CuZn superoxide dismutase, Mn superoxide dismutase,catalase and glutathione peroxidase in lymphocyte and erythrocytes in insulin-dependent diabetic children Bruno,H.;Stefan,I.;Mark,L.;Gosta,H.
  14. Horm. Metabol. Res. v.19 Erythrocyte lipid peroxidation and glutathione peroxidase activities in patients with diabetes mellitus Uzel,N.;Sivas,A.;Uysal,M.;Oz,H.
  15. Diabetes v.33 Superoxide dismutase in diabetic polymorphonuclear leukocytes Nath,N.;Chari,S.N.;Rathi,A.B.
  16. Ann. Rev. Biochem. v.52 Glutathione Meister,A.;Anderson,M.E.
  17. Methods in enzymol. v.53 Preparation and assay of superoxide dismutase Crapo,C.H.;McCord,J.M.;Fridovich,I.;Fleischer,S.(ed.);Packer,L.(ed.)
  18. Methods of Enzymatic Analysis(3rd ed.) v.2 Catalase Aebi,H.;Bergmeyer,H.U.(ed.);Bergmeyer,J.(ed.);GraB1,M.(ed.)
  19. Methods in enzymatic analaysis v.105 Assay of glutathione peroxidase Flohe,L.;Wolfgang,A.;Gunzler,W.A.;Packer,L.(ed.)
  20. Anal. Biochem. v.27 Enzymatic method for quantitative determination of nanogram anounts of total and oxidized glutathione Tietze,F.
  21. Methods in enzymol. v.113 γ-glutamyltranspeptidase from kidney Tate,S.S.;Meister,A.;Meister,A.(ed.)
  22. J. Biol. Chem. v.193 Protein measurement with folin phenol reagent Lowry,O.H.;Rosenbrough,NJ.;Farr,A.L.;Randall,R.J.
  23. Superoxide dismutase v.Ⅱ Production of superoxide radicals and hydrogen peroxide in mitochondria Boveris,A.;Cadenas,E.;Oberley L.W.(ed.)
  24. Ann. Rev. Physiol. v.48 Antioxidant defenses in the lung Fridovich,I.
  25. Diabetes v.36 Alterations in free radical tissue-defense mechanisms in streptozotocin-induced diabetes in rat.; Effect of insulin treatment Wohaieb,S.A.;Goddin,D.V.
  26. Proc. Natl. Acad. Sci. v.75 Evidence that the gamma-glutamyl cycle functions in vivo using intracellular glutathione: effects of amino acids and selective inhibition of enzymes Griffith,O.W.;Bridges,R.J.;Meister,A.
  27. Methods in enzymol. v.113 Glutathione biosynthesis:γ-glutamylcysteine synthetase from rat kidney Seelig,G.F.;Meister,A.;Meister,A.(ed.)
  28. J. Biol. Chem. v.254 Inhibition of glutathione biosynthesis by prothionine sulfoximine(S-n-propyl homocysteine sulfoximine), a selective inhibitor of gamma-glutamylcysteine synthetase Griffith,O.W.;Anderson,M.E.;Meister,A.
  29. J. Bio. Chem. v.254 Potent and specific inhibition of glutathione synthesis by buthionine sulfoximine (S-n-butyl homocysteine sulfoximine) Griffith,O.W.;Meister,A.
  30. Biochem. Pharmacol. v.30 Effect of selenium on glutathione metabolism. Induction of gamma-glutamylcysteine synthetase and glutathione reductase in the rat liver Chung,A.S.;Maines,M.D.