YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
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Isolation And Identification Of Soil Streptomyces sp. Producing An Immunomodulator That Restores Ultraviolet B Radiation-Induced Suppression Of The Immune Response

자외선에 의한 면역반응의 억제를 회복시키는 면역조절물질을 생산하는 토양 Streptomyces sp.의 분리 및 동정

  • Published : 1995.12.01
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Abstract

Soil microorganisms producing immunomoduators that can restore ultraviolet B (UVB) radiation-induced suppression of the immune response were screened in vitro. Exposure of freshly isolated murine epidermal cells (EC) to $180{\;}J/m^{2}$ of UVB radiation resulted in approximately 90% impairtnent of accessory cell function, as measured by their ability to support anti-CD3 monoclonal antibody-induced T-cell mitogenesis. When the culture supenmtants of 150 actinomycete strains were exanuned for their capacity to prevent or repair the UVB-induced impairment of accessory cell function, 4 of them were identified to contain immunomodulators that can restore the decreased accessory cell finiction. The soil isolate that showed the most effective restorative activity, G40025. was selected and fturther characters Addition of 10.mu.l of the culture supernatant of G40025 grown in G-media to cultures of UVB-irradiated EC right after UVB-irradiation restored the decreased accessory cell function by 58%. The immunomodtdator produced by G40025 appeared to be stable at 100.deg. C for 10 min. Taxonomical studies by cultural, morphological, and physiological characterization showed that the soil isolate, G40025, belongs to the genus Streptomyces.

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References

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