YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Water Transport during the Single-pass Perfusion Experiments : Effects of Some Perfusates and Their Osmolality

일회통과 관류실험시 물의 수송 : 관류액의 종류와 삼투압의 영향

  • 이정화 (대구효성카톨릭대학교 약학대학) ;
  • 이현주 (대구효성카톨릭대학교 약학대학) ;
  • 용철순 (영남대학교 약학대학) ;
  • 오두만 (대구효성카톨릭대학교 약학대학)
  • Published : 1995.08.01
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Abstract

The single-pass perfusion experiments were performed in anesthetized rats to investigate the effects of perfusates and their osmolality on the water transport and to determine the correlation between the extent of water transport and the volume change of perfusate. Phenol red was used as a nonabsorbable marker. In normal rats, when perfused at a flow rate of 0.5 ml/min, 2-(N-rnorpholino) ethanesulfonic acid (MES) and S$\phi$rensen's phosphate buffers showed minimal net water transport as 0.125 and 0.173 %/cm of intestinal length, respectively. Hypotonic perfusate of 200 mOsm/kg of water and hypertonic perfusate of 400 mOsm/kg of water generated significant water transport compared with isotonic perfusate of 300 mOsm/kg of water. There was a linear correlation between the extent of water transport and the volume change of perfusate, suggesting that the volume change can be used as a measure of water transport.

Keywords

References

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