Changes of Lectin from Viscum coloratum by Fermentation with Lactobacillus plantarum : Effect of pH and Temperature, Suger Specificity and Lymphocyte Stimulting Activity

유산균 발효에 의한 겨우사리 중의 렉틴 성분의 변화 : pH, 온도의 영향, 당 특이성, 림프구 자극분열효과

  • 박원봉 (서울여자대학교 자연과학대학) ;
  • 김희숙 (서울여자대학교 자연과학대학) ;
  • 나혜복 (서울여자대학교 자연과학대학) ;
  • 함승시 (강원대학교 농과대학)
  • Published : 1995.02.01


Lectin from mistletoe(Viscum coloratum) fermented by Lactobacillus plantarum was compared with the lectin from unfermented mistletoe. Agglunating activity of fermented mistletoe was stable at pH 3.77~8.71, at temperature range of $0~40^{\circ}C$ and in the presence of 9 mental ions, which results are similar to unfermented one, but less stable at pH 2.03~3.00 and more stable at temperature $60~80^{\circ}C$ than lectin from unfermented one. Agglunating activity of lectin from mistletoe fermented for 1 or 2 days and from fraction number 42~54 was not inhibited by all sugars used except for lectin from fraction number 21~34. Mitogenic activity to murine lymphpocytes of lectin from mistletoe was decreased by fermentation process.


  1. Oncology 43 v.1 no.SUP. Request an Impartial Discussion of the So-Called Mistletoe Therapy Franz,H.
  2. Oncology 43 v.1 no.SUP. Recent studies on the Anticancer Activities of Mistletoe(Viscum album) and Its alkaloids Tasneem,A;Khwaja,O.;Cecilia,B.;Stephanie,P.
  3. Oncology 43 v.1 no.SUP. Immunnomodulatory effects of Iscador; A Viscum album preparation Tibor,H.
  4. Oncology 43 v.1 no.SUP. Comparision of the effects of fermented and unfermented Mistletoe preparations of cultured tumor cell Gilles,R.G.;Jung,M.L.;Dominique,D.;Beck,J.P.
  5. Biochem. J. v.195 Isolation and properties of three lectins from mistletoe(Viscum album L.) Franz,H.;Ziska,P.;Kindt,A.
  6. J. Biol. Chem. v.257 no.22 Isolation and characterization of Viscumin, a toxic lectin from Viscum album L.(Mistletoe) Olsnes,S.;Stirpe,F.;Sandvig,K.;Pihl.A.
  7. Int. J. Biochem. v.11 The lectin from Viscum album L.-Isolation, characterization, properties and structure Luther,P.;Thesis,H.;Chatterjee,B.;Karduck,D.;Uhlenbruck,G.
  8. Annu. Rev. Immunol. v.3 Vitetta,E.S.;Uhr,J.W.
  9. Molecular action of Toxins and Viruses Olsnes,S.;Pihl,A.;Coben(ed.);von Heyningen(ed.)
  10. Biochem. Biophys, Acta. v.790 Bjorn,M.J.;Larrick,J.;Piatak,M.;Wilxon,K.J.
  11. Eur. J. Biochem. v.116 Thorpe,P.E.;Brown,A.N.F.;Ross,W.C.J.;Cumber,A.J.;Detre,S.I.;Edwards,D.C.;Davies,A.J.S.;Stirpe,F.
  12. FEBS Lett v.195 Ribosome-inactivating proteins up to date Fiorenzo S.;Luigi B.
  13. Immunology v.45 Stimulation of B cell but not T cell or thymocytes by a silialic acid-specific lectin Campbell,P.;Hartman,A.L.;Abel,C.A.
  14. Sci. Am. v.236 Lectins Sharon,N.
  15. 약학회지 v.38 no.4 겨우사리 중의 렉틴성분 분리 및 특성 박원봉;김희숙
  16. 약학회지 v.38 no.6 유산균 발효한 겨우사리 중의 렉틴 성분의 변화-분리 및 정제 박원봉;김희숙
  17. J. Biol. Chem. v.244 The reliability of the molecular weight determinations by dodecyl sufate-polyacrylamide gel electrophoresis Weber,K.;Osborn,M.
  18. L. Biol. Chem. v.260 Purification and characterization of an O-acetylsialin acid-specific lectin from marine crab Cancer atennarius Ravindranth,M.H.;Higa,H.H.;Cooper,E.L.;Paulson,J.C.
  19. J. Biol. Chem. v.258 A comparison of the mitogenic and nonmitogenic lectins from lima beans Pandofino,E.R.;Namen,A.E.;Munske,G.R.;Magnuson,J.A.