Parasites, Hosts and Diseases
- 제33권4호
- /
- Pages.387-390
- /
- 1995
- /
- 2982-5164(pISSN)
- /
- 1738-0006(eISSN)
이질아메바 한국분리주 YS-27의 무균 배양화
Axenization of Entamoeba histolytica, a Korean strain YS-27
- 장재경 (연세대학교 의과대학 열대의학연구소 및 기생충학교실) ;
- 임경일 (연세대학교 의과대학 열대의학연구소 및 기생충학교실) ;
- 소진탁 (연세대학교 의과대학 열대의학연구소 및 기생충학교실)
- Jang, Jae-Gyeong (Institute of Tropical Medicine & Department of Parasitology, Yonsei University College of Medicine) ;
- Im, Gyeong-Il (Institute of Tropical Medicine & Department of Parasitology, Yonsei University College of Medicine) ;
- So, Jin-Tak (Institute of Tropical Medicine & Department of Parasitology, Yonsei University College of Medicine)
- 발행 : 1995.12.01
초록
1969년 8월 10일 서울 고려병원의 입원친자 김00의 간농양에서 채취한 고름을 modified diphasic배지(Faust and Russell 1964)에서 배양하여 이필아메바 한국븐리쿠 YS-27을 얻었다. 이를 1985년 2월부터 TTY-53 배지에 넣어 Crithidia와 함께
In the present communication, YS-27, a Korean strain of Entnnloeba histoIWtica is described for the isolation and establishment of axonic cultivation. 5. histoLvticc, designated as strain "YS-27" was isolated from the pus of a hepatic abscess obtained from a 72 you old inpatient of August 10, 1969. Specimens, were obtained by needle aspiration, inoculated immediately and weekly cultured in a modified diphasic medium at 37℃. Strain YS-27 had been maintained for more than 15 years by weekly subculture until February, 1985. These cultures were transferred to a monoxenic TTY-SB medium seeded with a trypanosomatid of the genus CyithidiG. Penicillin G, 2 to 10 H 103 International units and Streptomycin, 2 to 10 mg per 100 ml, were added to the cultures to eliminate the bacteria. After more than one year later, these two organisms were well maintained by transfer every 3 or 4 days until .January. 1986 at 37℃ in TTY-SB medium in the absence of other microorganisms. These monoxenic cultures were then transferred to TYI-S-33 medium. Strain YS-27 alone had not been growing at the time of transfer, but when overlaid with Crithinia at intervals of 3 to 4 days, strain YS-27 propagated well. The Clthidio died out several weeks later after several passages. Beginning in April, 1986, strain YS-27. was successfully established in axonic culture in TYI-S-33 medium and has been maintained in continuous culture and multiplied well to present.