BMB Reports
- Volume 28 Issue 1
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- Pages.72-78
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- 1995
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- 1976-670X(eISSN)
Affinity Labeling of E. coli GTP Cyclohydrolase I by a Dialdehyde Derivative of Guanosine Triphosphate
- Ahn, Chi-Young (Department of Microbiology, College of Natural Sciences, Seoul National University) ;
- Park, Sang-Ick (Department of Microbiology, College of Natural Sciences, Seoul National University) ;
- Kim, Ju-Myeong (Department of Microbiology, College of Natural Sciences, Seoul National University) ;
- Yim, Jeong-Bin (Department of Microbiology, College of Natural Sciences, Seoul National University)
- Published : 1995.01.31
Abstract
Time-dependent inactivation of E. coli GTP cyclohydrolase I with a 2',3'-dialdehyde derivative of GTP (oGTP) was directed to the active site of the enzyme, and was dependent on the concentration of oGTP. The kinetics of inactivation were biphasic with a rapid reaction occurring immediately upon exposure of the enzyme to oGTP followed by a slow rate of inactivation. The