Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Inhibition of Micobial Growth by Paraquat

제초제 Paraquat의 미생물 생육저해 작용

  • Kim, Mi-Lim (Department of Food Science and Nutrition, Daegu Hyosung Catholic University) ;
  • Park, Chan-Sung (Department of Food Science, Kyoungsan University) ;
  • Choi, Kyoung-Ho (Department of Food Science and Nutrition, Daegu Hyosung Catholic University)
  • 김미림 (대구효성가톨릭대학교 식품영양학과) ;
  • 박찬성 (경산대학교 식품과학과) ;
  • 최경호 (대구효성가톨릭대학교 식품영양학과)
  • Published : 1995.08.31
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Abstract

This study was carried out to investigate the toxic action of herbicide, paraquat(1,1'-dimethyl-4,4'-bipyridylium-dichloride), against microorganisms. The toxic effect of paraquat was observed mainly using Escherichia coli(KCTC 1039), as follows; Growth of aerobic microorganisms which comprise 4 strains of bacteria and 2 strains of yeast and 4 strains of mold was inhibited drastically in the presence of 1.0mM paraquat But the growth of anaerobic bacteria was not affected by the chemical. When actively growing cells of E. coli were exposed to the paraquat at the concentration higher than 1.0 mM, they rapidly lost their ability to form colony and clearly formed inhibitory zone by well test More than 50% of the cells were killed by 1.0 mM paraquat treatment, even at immediate addition of paraquat to the medium.

시판 농약류의 미생물에 대한 독성을 검정한 결과 제초제인 paraquat(1,1'-dimethyl-4,4'-bipyridylium dichloride)가 편성혐기성 세균을 제외한 세균류와 효모 및 곰팡이에 걸치는 광범위한 미생물의 증식을 저해하였다. Paraquat는 $1.0\;{\mu}M$(0.186ppm)의 농도에서도 공시균(Escherichia coli KCTC 1039)의 turbidometric growth를 지연시켰으나, 생균수 변화 및 well test 등의 결과로부터 공시균에 대한 paraquat의 생육저지 최소 농도는 1.0mM(186ppm)로 평가되었다. Paraquat 처리시 공시균의 생균수는 처리직후에 대조구의 약 50%로 급격히 감소되었으나, 이후 4시간 동안 1.0mM의 paraquat 존재하에서도 유의적으로 증가되었다. 그러나 처리 4시간 이후로는 생균수가 서서히 감소되었으며 계속된 배양에도 불구하고 재증식은 관찰되지 아니하였다.

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