Applied Biological Chemistry
- 제38권1호
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- Pages.13-19
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- 1995
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
온도조절형(溫度調節型) 발현(發現) Vector를 함유한 Phenylalanine 생산균(生産菌)의 분자육종(分子育種)
Molecular Breeding of Phenylalanine Producing E. coli Containing Temperature-Controllable Vector
- Shim, Sang-Kook (Department of Food Technology, Dongnam Health Junior College) ;
- Lee, Young-Chun (Department of Food Science and Technology, Chung-Ang University) ;
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Chung, Ho-Kwon
(Department of Microbial Technology, Kon-Kuk University) ;
- Chung, Dong-Hyo (Department of Food Science and Technology, Chung-Ang University)
- 발행 : 1995.02.28
초록
Phenylalanine 생산시 tyrosine이 부생되지 않도록 tyrA유전자 발현이 낮은 여러 가지 온도조절형 plasmid를 제작하였으며, phenylalanine 생산균주인 대장균 AT2471
In order to produce phenylalanine without tyrosine co-production, we constructed various temperature-controllable expression vectors by insertion of lower expression of the tyrA gene into the plasmid pSY130-14. And tyrosine revertant to cultivate without addition of tyrosine, was selected from Escherichia coli strain AT2471[tyrA , thi ] by spontaneous mutation. The strain AT2471 harbouring plasmid pSY146A and the tyrosine revertant 5 harbouring plasmid pSY111-14 produced 12 g/l and 15 g/l of phenylalanine respectively in a 2.5 l jar fermenter at a constant temperature of