Applied Biological Chemistry

  • 제38권1호
  • /
  • Pages.7-12
  • /
  • 1995
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  • 2468-0834(pISSN)
  • /
  • 2468-0842(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지

효모(酵母) 유전자(遺傳子) 발현용(發現用) Promoter 개발(開發)에 관(關)한 연구(硏究)

Studies on the Development of Yeast Promoter for the Gene Expression

  • 정호권 (건국대학교 공과대학 미생물학과) ;
  • 박준희 (중앙대학교 산업대학 식품가공학과) ;
  • 심상국 (동남보건전문대학 식품가공과) ;
  • 정동효 (중앙대학교 산업대학 식품가공학과)
  • Chung, Ho-Kwon (Department of Microbial Technology, Kon-Kuk University) ;
  • Park, Joon-Hee (Department of Food Science and Technology, Chung-Ang University) ;
  • Shim, Sang-Kook (Department of Food Technology, Dongnam Health Junior College) ;
  • Chung, Dong-Hyo (Department of Food Science and Technology, Chung-Ang University)
  • 발행 : 1995.02.28
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초록

본 연구는 lacZ' 유전자의 promoter 개발을 위하여 착수하였다. lacZ' 유전자의 heterologous promoter I과 II를 효모 염색체의 Bam HI DNA 단편에서 분리하였다. Promoter I의 크기는 2.5 Kb 정도이고 ${\beta}-galactosidase$ 활성은 124.6 U/mg protein이었으며 promoter II의 크기와 효소활성은 4.0 Kb와 168.8 U/mg이었다. 형질 전환체에서의 YEp plasmid 안정성은 52.7%에서 67.4% 정도였다. YEp plasmid로부터 YIp plasmid를 재조합하였으며 이 YIp plasmid는 대장균에서나 효모에서도 발현되었다. 효모로부터 분리한 promoter I과 II는 재조합된 YEp와 YIp plasmid의 promoter로서 이용 가능하였다.

The purpose of this study was the development of promoter for the lacZ' gene. Two heterologous promoter I and II of lacZ' gene were isolated from chromosomal DNA Bam HI fragment of yeast. The size of the promoter I was estimated to be 2.5 kb and ${\beta}-galactosidase$ activity was 124.6 U/mg protein, and the size of the promoter II was 4.0 kb and its ${\beta}-galactosidase$ activity was 168.8 U/mg protein, respectively. The stability of the recombinant YEp plasmid in the transformant was from 52.7 to 67.4% at minimal medium. YIp plasmid was constructed from YEp plasmid, and expressed both in E. coli and yeast. The promoter I aid II iso-lated from yeast chromosomal DNA can be used for promoter of plasmid YEp and YIp.

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