Korean Journal of Food Science and Technology (한국식품과학회지)
- Volume 27 Issue 3
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- Pages.370-374
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- 1995
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- 0367-6293(pISSN)
Selective Removal of Protease from Soybean Cell Wall Degrading Enzyme Complex Isolated from Aspergillus niger CF-34
Aspergillus niger CF-34로부터 분리한 대두세포벽분해효소 복합체 중의 Protease의 선택적인 제거
- Choi, Yeon-Bae (Dr. Chung's Food Co., Ltd., Central Research Institute) ;
- Kim, Kang-Sung (Dr. Chung's Food Co., Ltd., Central Research Institute) ;
- Sohn, Heon-Soo (Dr. Chung's Food Co., Ltd., Central Research Institute)
- Published : 1995.06.30
Abstract
By exposing the complex enzyme solution to alkaline condition, it was possible to remove the protease activity selectively without inactivation of soybean cell wall degrading activity of the crude enzyme complex produced by Aspergillus niger CF-34. Optimum reaction conditions were as follow. pH was
Aspergillus niger CF-34가 생산하는 대두세포벽분해 효소 복합액을 알칼리로 처리하여 조효소액 중에 함유된 protease만을 선택적으로 제거할 수 있었다. 조효소액을 알칼리로 처리하였을 때 세포벽분해활성에는 영향을 적게 주고, 대두 단백질을 분해시켜 쓴 맛의 peptide를 생성하는 protease만을 선택적으로 불활성화시킬 수 있었다. 조효소액의 pH를