Analysis of Taxol and Cephalomannine in Cheju Island Yew (Taxus cuspidata S. et Z.)

제주도 주목(Taxus cuspidata S. et Z.)의 Taxol 및 Cephalomannine의 함량분석

  • Kwak, Sang-Soo (Bioresources Research Group, Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Choi, Myung-Suk (Department of Forestry, Kyungpook National University) ;
  • Park, Young-Goo (Department of Forestry, Kyungpook National University) ;
  • Ko, Kyung-Soo (Development & Research Team, Hankook Shin Yak Pharm. Co., LTD.) ;
  • Liu, Jang-Ryol (Bioresources Research Group, Korea Research Institute of Bioscience and Biotechnology, KIST)
  • 곽상수 (KIST 생명공학연구소 생물자원연구그룹) ;
  • 최명석 (경북대학교 임학과) ;
  • 박용구 (경북대학교 임학과) ;
  • 고경수 ((주) 한국신약 개발연구실) ;
  • 유장렬 (KIST 생명공학연구소 생물자원연구그룹)
  • Published : 1995.06.30

Abstract

To seek for a stable supply source of taxol as an anticancer agent urgently needed for the treatment of ovarian cancer, the concentrations of taxol and cephalomannine in bark and needles of 37 yew (Taxus cuspidata S. et Z.) trees grown in 6 locations of Cheju Island were analyzed by using HPLC. A considerable variation in the content was observed in plant part and the location. The average taxol content in the needles ($220.3{\;}{\pm}{\;}56{\;}{\mu}g$ per g dry wt) was ca. 4 times hgher than that in the bark, whereas the content of cephalomannine in the needles $(353{\;}{\pm}{\;}141{\;}{\mu}g)$ was ca. 5 times higher than that in the bark. Particularly, even within Cheju Island, needles from trees grown near the coast including Sogwipo area contained much higher taxol contents than needles from trees grown at mountain locations, indicating that taxane level is affected by environmental effects. Taxol in the needles was confirmed by electrospray mass spectrometry. These results suggest that the needles of yew trees growing in Cheju Island may provide a renewable source for taxol.

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