Solid-culture of Lepista nuda

Lepista nuda의 고체배양

  • Lee, Sang-Sun (Graduate School, Korea National University of Education) ;
  • Choi, Kyoung-Jin (Graduate School, Korea National University of Education)
  • 이상선 (한국교원대학교 대학원, 생물과학 및 생물교육) ;
  • 최경진 (한국교원대학교 대학원, 생물과학 및 생물교육)
  • Published : 1995.06.30

Abstract

The mycelia of Lepista nuda were isolated from the different basidiocarps, collected from the forests of Robinia pseudo-acaciae and mixed forests of R. pseudo-acaciae and Quercus serrata. On the different woods, the growths of mycelia collected were compared with those transferred from Applied Mycology Division, RDA. Also, the measurements of dry weight loss, as growth of mycelia, were correlated to the productions of carbon dioxide on the different wood substrates. The growth of mycelia was observed to be best on the woods of R. pseudo-acaciae; followed by the growth on the woods of Morus alba. The growth rate of mycelia appeared to be various, depending on the different kinds of wood substrates. The soild-cultures of the mycelia on the sawdust of the R. pseudo-acaciae woods and the grains of sorghum and corn were conducted, but the basidiocarps were not obtained from these. Therefore, this mycelium collected was believed to be important as new mushroom utilizing the different woods resources instead of Q. serrata.

한국교원대학교 주변의 아카시아림 및 상수리나무와 아카시아의 혼합림에서 민자주방망이버섯(L. nuda)을 채집하여 균사를 순수분리하였다. 분리된 균사와 농진청 균이과에서 분양 받은 균사를 시료로 수종의 기질에 따른 균사의 성장을 나무 건중량과 탄산가스 발생량으로 측정하였다. 이 결과 아카시아, 뽕나무, 느티나무 순으로 균사의 성장을 나타냈다. 아카시나무 톱밥, 조 및 옥수수를 이용한 고형발효를 한 결과 균사의 성장에 관련된 성장곡선은 그렸으나, 자실형성을 하지 못하였다. 그러나, 앞으로 유망한 식용버섯으로 다른 수종의 나무에 자라는 균사를 얻었으며, 자실체 형성을 위하여서는 많은 연구가 필요하다.

Keywords

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