The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Screening for Phyllospheral Antagonistic Microorganisms for Control of Red-pepper Anthracnose (Collectotrichum gloeosporioides)

고추탄저병(Colletotrichum gloeosporioides) 방제를 위한 엽권 길항미생물의 탐색

  • Paik, Su-Bong (Department of Agronomy, College of Agriculture, Kon-Kuk University) ;
  • Kim, Dong-Woo (Department of Agronomy, College of Agriculture, Kon-Kuk University)
  • 백수봉 (건국대학교 농과대학 농학과) ;
  • 김동우 (건국대학교 농과대학 농학과)
  • Published : 1995.06.30
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Abstract

For the screening of biocontrol agents against red-pepper anthracnose (Colletotrichum gloeosporioides Penz) 248 isolates of bacteria, 51 of fungi and 30 of yeasts were obtained from phyllospere of medicinal plants. Of isolated microorganisms, four bacterial isolates, KB6, KB12, KB13 and KB14 were highly antagonistic to C. gloeosporioides than the others through dual culture test on potato dextrose agar (PDA). Among the four bacterial isolates, culture filtrate of the isolate KB12 showed the highest inhibition of C. gloeosporioides on PDA. The culture filtrates of four isolates controlled anthracnose on the red fruits, but not on the green fruits. In the living bacterial cell test, high control effect was observed both on the red and the green fruits. In the biochemical test, all isolates were identified as Bacillus subtilis.

고추탄저병(Colletotrichum gloeosporioides)에 대한 생물학적방제 능력을 가지는 엽권미생물을 선발하기 위하여 38과 87종의 약용식물로부터 243균주의 세균과 51균주의 사상균 그리고 30균주의 효모를 분리하여 이들에 대하여 항진균활성검정을 in vitro 및 in vivo에서 실시한 결과는 다음과 같다. 분리된 엽권미생물 중에서 PDA 배지 대치배양에서 10 mm 이상의 생육저지대를 형성하는 4개의 엽권미생물이 선발되었다. 선발된 4개의 세균균주 KB6, KB12, KB13 및 KB14의 PDB 배양여액 중에서 KB12 균주가 균사생장 억제율이 가장 높았다. 액체배양여액 처리에서 푸른 고추보다 붉은 고추에서 방제효과가 컸다. Bacterial cell 처리에서는 푸른 고추나 붉은 고추에서도 높은 방제효과가 있었다. 선발된 4개의 세균균주들은 모두 Bacillus subtilis로 동정되었다.

Keywords

References

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