Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
권호 표지

Development Rates of the Cryopreserved Mouse Embryos According to the Embryonic Stage and Cryopreservation Method

생쥐 초기배아의 발생시기와 냉동보존 방법에 따른 발생률

  • Cheon, Yong-Pil (Dmepartent of Biology, College of Natural Sciences, Hanyang University) ;
  • Lee, Ho-Jun (IVF Research Laboratory, Cheil General Hospital) ;
  • Kim, Moon-Kyoo (Dmepartent of Biology, College of Natural Sciences, Hanyang University)
  • 전용필 (한양대학교 자연과학대학 생물학과) ;
  • 이호준 (제일병원 체외수정연구실) ;
  • 김문규 (한양대학교 자연과학대학 생물학과)
  • Published : 1994.12.26
Download PDF
⟨ Previous Next ⟩

Abstract

The study has been carried out in order to evaluate the effects of embryonic stage, and cryopreservation method on the rates of viability and development of the cryopreserved mouse early embryos. The results were as following:In the treatment steps of cryoprotectant, for the fertilized oocyte with pronucleus(PN), 2-step was better than the others. And for the other embryos, 4-step was better than 2- or 3-step. In respect to the embryonic stage, as the embryos developed from fertilized oocytes to 8-cell embryos, the rates of viability and development were increased higher. Therefore, 8-cell embryo was better stage than the others. In respect to the kind of cryoprotectants, PROH was better than DMSO for the fertilized oocyte, as a cryoprotectant. DMSO, for the 2-cell embryos and PROH and DMSO for the 4- and 8-cell embryos were suitable for cryopreservation.

Keywords