Clinical and Experimental Reproductive Medicine
- Volume 21 Issue 3
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- Pages.285-296
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- 1994
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
The Effect of $Ca^{2+}$ and Its Specific Time in the Maturation of Mammalian Oocyte
포유류의 난자성숙과 성숙과정에서 $Ca^{2+}$ 의 영향과 그 작용시기
- Bae, In-Ha (Department of Bilogy, College of Natural Sciences, Sungshin Women's University)
- 배인하 (성신여자대학교 생물학과)
- Published : 1994.12.26
Abstract
Follicular oocytes were released from the graafian follicles of ovaries from 3-4 weeks old mice. The spontaenous maturation of these follicular oocyes was inhibited by the treatment of dbcAMP and progesterone and these oocytes were cultured for 2-8hr in the Modified Hank's balanced salt solution(MHBS). Ethylenediaminetetraaceticacid(EDTA) and calmoudulin antagonist, trifluoperazine (TFP) were treated to the culture medium in order to investigate whether these chemical agents inhibit calcium uptake into the oocyte and oocyte maturation.
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