Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Genetic Organization of the Recombinant Bacillus pasteurii Urease Genes Expressed in Escherichia coli

  • Kim, Sang-Dal (Department of Applied Microbiology, Yeungnam University) ;
  • Hausinger, Robert P. (Departments of Microbiology and Biochemistry, Michigan State University)
  • Published : 1994.06.01
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Abstract

The genetic organization of the urease gene cluster from an alkalophilic Bacillus pasteurii was determined by subcloning and Tn5 transposon mutagenesis of a 10.7 kilobasepair cloned fragment. A region of DNA between 5.0 and 6.0 kb in length is necessary for urease activity. In vitro transcription-translation analysis of transposon insertion mutants of the cloned urease genes demonstrated that the major ($M_r$ 67,000) and minor ($M_r$ 20,000) structural peptides of urease are encoded at one end of the urease gene cluster and at least 3 additional polypeptides are encoded by adjacent DNA sequences.

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